Bradford Protein Content Assay Kit
Bradford法蛋白含量检测试剂盒
货号:AKPR015
规格: 500T
检测设备:可见分光光度计/酶标仪
可检测样本数:500 Samples
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Product Information
Bradford Protein Content Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:500 Samples
  • 主要检测设备及配套:可见分光光度计(1 mL玻璃比色皿)/酶标仪(96孔板)
  • 预计测定时间:6 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
考马斯亮蓝G-250染料游离状态下呈棕红色,与蛋白质结合后变为蓝色,染料的最大吸收峰由465 nm变为595 nm,在一定浓度范围内蛋白质-染料复合物吸光值与蛋白质含量成正比,通过测定595 nm处吸光值的变化即可定量检测蛋白质的含量。
  • 检测方法: Bradford
  • 检测波长: 595 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: BSA
  • 参考标准: y=3.9836x+0.8988 (R2=0.9997)
  • 标准线性范围: 0.04-0.2 mg/mL
  • 检测限: 0.02 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
① Bradford法测定蛋白浓度不受绝大部分样品中的化学物质的影响,样品中巯基乙醇的浓度可高达1 M,二硫苏糖醇的浓度可高达5 mM;但受略高浓度的去垢剂影响,需确保样品中SDS浓度低于0.1%,TritonX-100低于0.1%,Tween 20、60、80低于0.06%;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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[4] Lin D, Zhuang Z, Yu N, et al. Comprehensive effects of microplastics on algae-laden surface water treatment by coagulation-ultrafiltration combined process: Algae cultivation, coagulation performance and membrane fouling development[J]. Science of The Total Environment, 2024: 171553. (IF 9.8)

[5] Qiao Y, Shen Y, Jiang H, et al. Structural characterization, antioxidant and antibacterial activity of three pectin polysaccharides from blueberry[J]. International Journal of Biological Macromolecules, 2024: 129707.(IF 8.2)

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[7] Du P, Song J, Qiu H, et al. Polyphenols Extracted from Shanxi-Aged Vinegar Inhibit Inflammation in LPS-Induced RAW264. 7 Macrophages and ICR Mice via the Suppression of MAPK/NF-κB Pathway Activation[J]. Molecules, 2021, 26(9): 2745.(IF 4.412)

[8] Du P, Li N, Wang J, et al. Structural Characterization and Antifatigue Activity of a Novel Exopolysaccharide Isolated from Marasmius androsaceus[J]. Journal of Food Biochemistry, 2023, 2023.(IF 4)

[9] Cheng H, Chang S, Shi X, et al. Molecular Mechanisms of the Effects of Sodium Selenite on the Growth, Nutritional Quality, and Species of Organic Selenium in Dandelions[J]. Horticulturae, 2024, 10(3): 209. (IF 3.1)

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