BCA Protein Content Assay Kit
BCA法蛋白含量检测试剂盒
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Product Information
BCA Protein Content Assay Kit
-20℃ Dry Ice Transportation
- 检测样本量：100 Samples
- 主要检测设备及配套：可见分光光度计（1 mL玻璃比色皿）/酶标仪（96孔板）
- 预计测定时间：3 h (100 Samples)
- 试剂储存条件：按照标签要求储存
-
需自备试剂:无
Detection Principle
BCA（Bicinchonininc Acid）蛋白含量检测法是用于总蛋白质定量的常用方法，BCA与二价铜离子混合即为BCA工作液，蛋白在碱性条件下能够将Cu2+还原为Cu+，Cu+与BCA试剂可形成紫色络合物，产物在562 nm处具有特征吸收峰，通过吸光值变化即可定量检测样品的蛋白浓度。
- 检测方法： BCA
-
检测波长： 562 nm
-
信号响应： 递增型
Refreence Information
- 标准物质： BSA
- 参考标准： y=1.8946x+0.1219 (R2=0.9996)
- 标准线性范围： 0.1-0.5 mg/mL
- 检测限： 0.05 mg/mL
- 注：不同仪器及比色材质会对结果产生影响，以实际测定值为准。
Notices
①Copper Solution与 PBS Diluent可置于2-8℃长期保存，若发现污染浑浊则应丢弃；BCA Solution在低温条件下出现结晶沉淀时，可37℃温育使其完全溶解，不影响使用；
②为了您的安全和健康，请穿实验服并戴一次性手套操作；
③BCA法测定蛋白含量不受绝大部分样品中化学物质的影响，样品中5% SDS、5% Triton X-100、5% Tween20、60、80等常用浓度去垢剂不影响检测结果，但EDTA和EGTA等螯合剂、DTT和巯基乙醇等还原剂和脂类会影响检测结果，需确保EDTA低于10 mM、无EGTA、二硫苏糖醇低于1 mM、巯基乙醇低于0.01%；若样品含有较多干扰物质且本身背景值较高，建议使用Bradford法蛋白含量检测试剂盒（Cat AKPR015）。
注： 为保证结果准确且避免试剂损失，测定前请仔细阅读说明书（以实际收到说明书为准），确认试剂储存和准备是否充分，操作步骤是否清楚，且务必取2-3个预期差异交的样本进行预测定，过程中问题请您及时与工作人员联系。
Product Citation
[1] Zhang P, Zhou C, Ren X, et al. Inhibiting the compensatory elevation of xCT collaborates with disulfiram/copper-induced GSH consumption for cascade ferroptosis and cuproptosis[J]. Redox Biology, 2023: 103007.(IF 11.4)
[2] Yi Y, Li W, Liu K, et al. Licorice-saponin A3 is a broad-spectrum inhibitor for COVID-19 by targeting viral spike and anti-inflammation[J]. Journal of Pharmaceutical Analysis, 2023.(IF 8.8)
[3] Gao Y, Jin F, Zhang P, et al. Elesclomol-copper synergizes with imidazole ketone erastin by promoting cuproptosis and ferroptosis in myelodysplastic syndromes[J]. Biomedicine & Pharmacotherapy, 2024, 175: 116727. (IF 7.5)
[4] Zhou C, Wang L, Hu W, et al. CDC25C is a prognostic biomarker and correlated with mitochondrial homeostasis in pancreatic adenocarcinoma[J]. Bioengineered, 2022, 13(5): 13089-13107.(IF 6.832)
[5] Xu J, Zhao N, Meng X, et al. Contribution of amino acids to Alicyclobacillus acidoterrestris DSM 3922T resistance towards acid stress[J]. Food Microbiology, 2023, 113: 104273.(IF 5.3)
[6] Wang L, Wang H, Yang C, et al. Investigating CENPW as a Novel Biomarker Correlated With the Development and Poor Prognosis of Breast Carcinoma[J]. Frontiers in Genetics, 2022, 13: 900111.(IF 4.772)
[7] Liu Z Y, Yang H L, Cai G H, et al. LTA and PGN from Bacillus siamensis can alleviate soybean meal-induced enteritis and microbiota dysbiosis in Lateolabrax maculatus[J]. Fish & Shellfish Immunology, 2024: 109618. (IF 4.7)
[8] Du P, Song J, Qiu H, et al. Polyphenols Extracted from Shanxi-Aged Vinegar Inhibit Inflammation in LPS-Induced RAW264. 7 Macrophages and ICR Mice via the Suppression of MAPK/NF-κB Pathway Activation[J]. Molecules, 2021, 26(9): 2745.(IF 4.412)
[9] Wang G, Zhang X, Du G, et al. Oleic Acid and Linoleic Acid Enhances the Biocontrol Potential of Metarhizium rileyi[J]. Journal of Fungi, 2024, 10(8): 521. (IF 4.2)
[10] Du P, Li N, Wang J, et al. Structural Characterization and Antifatigue Activity of a Novel Exopolysaccharide Isolated from Marasmius androsaceus[J]. Journal of Food Biochemistry, 2023, 2023.(IF 4)
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The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
This equation is commonly abbreviated as: C1V1 = C2V2
Sample(S):可测定的样品数量；
★根据此数量计算需要的试剂盒个数。
Size
120-600T
Test(T):总反应次数；
★每个试剂盒均预留了至少5个（分光光度法）和10个（微板法）样本预实验的试剂用量；
★若需要制作标准曲线则至少能够满足2次制作标准曲线的试剂用量（15个）。
