L-Lactic Acid (L-LA) Content Assay Kit (WST-1 Method)
L-乳酸含量检测试剂盒(WST-1法)
货号:AKAC001-1C
规格: 70T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
L-Lactic Acid (L-LA) Content Assay Kit (WST-1 Method)
-20℃ Dry Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:4 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
L-乳酸脱氢酶(L-LDH)能够催化L-乳酸生成丙酮酸,同时使NAD+还原生成NADH和H+,进一步通过1-mPMS的递氢作用,还原WST-1生成水溶性甲臜,产物在450 nm处具有特征吸收峰,通过吸光值变化即可定量检测L-乳酸的含量。
  • 检测方法: WST-1法
  • 检测波长: 450 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: L-Lactic Acid
  • 参考标准: y=1.6821x+0.0168 (R2=0.9997)
  • 标准线性范围: 0.025-0.8 µmol/mL
  • 检测限: 0.025 µmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①若A测定或∆A测定超出标准吸光值线性范围:高于最高值建议将待测样本使用蒸馏水适当稀释后再进行测定;低于最低值建议制备更高浓度样本后再进行测定,计算时相应修改;

②试剂三配制后有效期较短,为便于试验安排,附赠一瓶试剂三作为备用,每瓶均可完成至少25个样本的检测用量;

③提取液中含有蛋白沉淀组分,待测样本不能用于蛋白浓度的测定,若需要使用蛋白浓度计算结果,建议使用PBS或生理盐水制备样本后再进行蛋白浓度测定;

④反应时间对实验结果有较大影响建议精确控制,测定样本较多时建议分批进行测定,以避免组内反应时间不一致对结果造成影响;

⑤反应体系中试剂不能按比例配制为混合液使用,必须按反应体系顺序依次加入;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Liu B, Xian Y, Chen X, et al. Inflammatory Fibroblast‐Like Synoviocyte‐Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis[J]. Advanced Science, 2024: 2307338.(IF 15.1)

[2] He Y, Jiang S, Zhong Y, et al. USP7 promotes non‐small‐cell lung cancer cell glycolysis and survival by stabilizing and activating c‐Abl[J]. Clinical and Translational Medicine, 2023, 13(12): e1509.(IF 10.6)

[3] Liu W X, Wang J J, Xiao X K, et al. Antimicrobial effects and metabolomics analysis of cell-free supernatant produced by Pediococcus acidilactici LWX 401 isolated from Yunnan traditional pickles[J]. LWT, 2024, 191: 115626.(IF 6)

[4] Zhou T, Xiao Z, Lu J, et al. IGF2BP3-mediated regulation of GLS and GLUD1 gene expression promotes treg-induced immune escape in human cervical cancer[J]. American Journal of Cancer Research, 2023, 13(11): 5289.(IF 5.3)

[5] Yu J, Jiang W, Wang S, et al. Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora[J]. Journal of Functional Foods, 2022, 97: 105255.(IF 5.223)

[6] Du P, Li N, Wang J, et al. Structural Characterization and Antifatigue Activity of a Novel Exopolysaccharide Isolated from Marasmius androsaceus[J]. Journal of Food Biochemistry, 2023, 2023.(IF 4.0)

[7] Cui H, Ma Y, Han S, et al. Arsenic trioxide regulates the glycolytic pathway to treat acute promyelocytic leukemia by inhibiting RPL22L1[J]. Leukemia Research, 2024: 107550. (IF 2.1)

[8] Li P, Tang C, Fu D, et al. Cardamonin suppresses glycolysis and induces oxidative stress by inhibiting PI3K/AKT/mTOR pathway in bladder cancer cells[J]. Tropical Journal of Pharmaceutical Research, 2023, 22(8): 1541-1546.(IF 0.6)

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