①探针装载后,一定要洗净残余的未进入细胞内的探针,否则会导致背景较高;
②探针装载完毕并洗净残余探针后,可以进行激发波长的扫描和发射波长的扫描,以确认探针的装载情况是否良好;
③尽量缩短探针装载后到测定所用的时间(刺激时间除外),以减少各种可能的误差;
④为了您的安全和健康,请穿实验服并戴一次性手套操作;
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