①探针装载后,一定要洗净残余的未进入细胞内的探针,否则会导致背景较高;
②DHE在光照和空气中易被氧化,保存和操作中应注意避光;
③若需要设置无DHE对照,即不加探针,只使用PBS重悬的细胞;
④为了您的安全和健康,请穿实验服并戴一次性手套操作;
[1] Huang Y, Kou Q, Su Y, et al. Combination therapy based on dual-target biomimetic nano-delivery system for overcoming cisplatin resistance in hepatocellular carcinoma[J]. Journal of Nanobiotechnology, 2023, 21(1): 89.(IF 10.2)
[2] Xie J, Hu Y, Su W, et al. PLGA nanoparticles engineering extracellular vesicles from human umbilical cord mesenchymal stem cells ameliorates polyethylene particles induced periprosthetic osteolysis[J]. Journal of Nanobiotechnology, 2023, 21(1): 398.(IF 10.2)
[3] Ji C, Miao J, Xia B, et al. Evaluation of the toxic effects of fluindapyr, a novel SDHI fungicide, to the earthworms Eisenia fetida[J]. Science of The Total Environment, 2023, 899: 165697.(IF 9.8)
[4] Xie J, Hu Y, Li H, et al. Targeted therapy for peri-prosthetic osteolysis using macrophage membrane-encapsulated human urine-derived stem cell extracellular vesicles[J]. Acta Biomaterialia, 2023, 160: 297-310.(IF 9.7)
[5] Li Y, Li C, Liu S, et al. Nano-photosensitizers with gallic acid-involved Fe–O–Cu “electronic storage station” bridging ligand-to-metal charge transfer for efficient catalytic theranostics[J]. Journal of Colloid and Interface Science, 2024. (IF 9.4)
[6] Xian Y, Gao Y, Su Y, et al. Cichoric acid targets RANKL to inhibit osteoclastogenesis and prevent ovariectomy‐induced bone loss[J]. Phytotherapy Research, 2024. (IF 7.2)
[7] Liang X, Li Y, Wang L, et al. Knockout of stigmatic ascorbate peroxidase 1 (APX1) delays pollen rehydration and germination by mediating ROS homeostasis in Brassica napus L[J]. The Plant Journal, 2024. (IF 7.2)
[8] Zhu Y, Lin K, Zhang X, et al. Antibacterial Effect of Gallic Acid in UV-C Light Treatment Against Escherichia coli O157: H7 and the Underlying Mechanism[J]. Food and Bioprocess Technology, 2023: 1-16.(IF 5.6)
[9] Du P, Li N, Wang J, et al. Structural Characterization and Antifatigue Activity of a Novel Exopolysaccharide Isolated from Marasmius androsaceus[J]. Journal of Food Biochemistry, 2023, 2023.(IF 4)