Reactive Oxygen Species (ROS)Assay Kit
活性氧检测试剂盒
货号:AKCE002-2
规格: 100-500T(红色荧光)
检测设备:荧光分光光度计
可检测样本数:100-500 Samples
In Stock
BOXBIO提供专业的指标检测服务,如需检测服务,请点击右侧按钮
检测服务
Product Information
Reactive Oxygen Species (ROS)Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:100-500 Samples
  • 主要检测设备及配套:荧光显微镜/荧光分光光度计/酶标仪或流式细胞仪检测
  • 预计测定时间:-
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
利用荧光探针DHE进行活性氧检测是一种基于荧光强度变化,检测细胞内活性氧水平的经典方法,DHE可以自由穿过细胞膜,进入细胞内后,在细胞质中呈蓝色荧光,被细胞内的ROS氧化后形成氧化乙啶掺入染色体DNA中,使细胞核呈现明亮红色荧光,在激发波长535 nm,发射波长610 nm处,使用荧光显微镜、荧光分光光度计、流式细胞仪或激光共聚焦显微镜等检测荧光信号,测定细胞内活性氧水平。
  • 检测方法: DHE探针法
  • 检测波长: 535 nm & 610 nm
  • 信号响应: 递增型
Notices

①探针装载后,一定要洗净残余的未进入细胞内的探针,否则会导致背景较高;

②DHE在光照和空气中易被氧化,保存和操作中应注意避光;

③若需要设置无DHE对照,即不加探针,只使用PBS重悬的细胞;

④为了您的安全和健康,请穿实验服并戴一次性手套操作;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Huang Y, Kou Q, Su Y, et al. Combination therapy based on dual-target biomimetic nano-delivery system for overcoming cisplatin resistance in hepatocellular carcinoma[J]. Journal of Nanobiotechnology, 2023, 21(1): 89.(IF 10.2)

[2] Xie J, Hu Y, Su W, et al. PLGA nanoparticles engineering extracellular vesicles from human umbilical cord mesenchymal stem cells ameliorates polyethylene particles induced periprosthetic osteolysis[J]. Journal of Nanobiotechnology, 2023, 21(1): 398.(IF 10.2)

[3] Ji C, Miao J, Xia B, et al. Evaluation of the toxic effects of fluindapyr, a novel SDHI fungicide, to the earthworms Eisenia fetida[J]. Science of The Total Environment, 2023, 899: 165697.(IF 9.8)

[4] Xie J, Hu Y, Li H, et al. Targeted therapy for peri-prosthetic osteolysis using macrophage membrane-encapsulated human urine-derived stem cell extracellular vesicles[J]. Acta Biomaterialia, 2023, 160: 297-310.(IF 9.7)

[5] Li Y, Li C, Liu S, et al. Nano-photosensitizers with gallic acid-involved Fe–O–Cu “electronic storage station” bridging ligand-to-metal charge transfer for efficient catalytic theranostics[J]. Journal of Colloid and Interface Science, 2024. (IF 9.4)

[6] Xian Y, Gao Y, Su Y, et al. Cichoric acid targets RANKL to inhibit osteoclastogenesis and prevent ovariectomy‐induced bone loss[J]. Phytotherapy Research, 2024. (IF 7.2)

[7] Liang X, Li Y, Wang L, et al. Knockout of stigmatic ascorbate peroxidase 1 (APX1) delays pollen rehydration and germination by mediating ROS homeostasis in Brassica napus L[J]. The Plant Journal, 2024. (IF 7.2)

[8] Zhu Y, Lin K, Zhang X, et al. Antibacterial Effect of Gallic Acid in UV-C Light Treatment Against Escherichia coli O157: H7 and the Underlying Mechanism[J]. Food and Bioprocess Technology, 2023: 1-16.(IF 5.6)

[9] Du P, Li N, Wang J, et al. Structural Characterization and Antifatigue Activity of a Novel Exopolysaccharide Isolated from Marasmius androsaceus[J]. Journal of Food Biochemistry, 2023, 2023.(IF 4)

Related Products
Publication Reward
BOXBIO 助研基金申请
JOIN
*发表文章中注明Boxbio、北京盒子生工科技有限公司(Beijing Boxbio Science & Technology Co.,Ltd.) 即可申请。
Calculator
The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
  • x
    =
    x
Make Science Easy
Personal Assistant
BoxLab Provides Butler service
Professional Technical support
Product Innovation Protocol
Quality Control
Box QC&AS Provides
Strict Quality Management Process
Considerate After Sales service
Professional Service
Box Customer Service Provides
Pre-sales Service
Product ordering and cooperation
北京盒子生工科技有限公司旗下产品
版权所有:Copyright(C)2020 北京盒子生工科技有限公司