Lignin Content Assay Kit
木质素含量检测试剂盒
货号:AKSU010U
规格: 60T/50S
检测设备:紫外分光光度计
可检测样本数:50 Samples
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Product Information
Lignin Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL石英比色皿(d=10 mm)
  • 预计测定时间:2 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    高氯酸(HClO4,MW = 100.46,CAS: 7601-90-3);
    冰乙酸(C2H4O2,MW = 60.05,CAS: 64-19-7)
Detection Principle
木质素中的酚羟基发生乙酰化后生成乙酰木质素,产物在280 nm处具有特征吸收峰,通过吸光值变化即可定量检测木质素的含量。
  • 检测方法: 乙酰化法
  • 检测波长: 280 nm
  • 信号响应: 递增型
Notices

①应适当调整冰乙酸加入量,使A测定处于0.1-0.8之间可提高结果准确性,计算时相应修改;

②试剂一和冰乙酸具有刺激性和挥发性,高氯酸具有强腐蚀性,操作时请做好防护措施;

③加热过程需严格密封,以防气体溢出,加热过程伴随有剧烈反应,混匀时应缓慢轻摇,以免压力过大喷出造成伤害;

④若玻璃试管操作不方便,可使用带盖密封性较好的离心管作为反应容器,请确保无橡胶等易腐蚀材质,以免造成泄漏;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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