Glycolate Oxidase (GO) Activity Assay Kit
乙醇酸氧化酶活性检测试剂盒
货号:AKPL002U
规格: 60T/50S
检测设备:紫外分光光度计
可检测样本数:50 Samples
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Product Information
Glycolate Oxidase (GO) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL石英比色皿(d=10 mm)
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
乙醇酸氧化酶催化乙醇酸氧化生成乙醛酸,乙醛酸能够与盐酸苯肼反应生成乙醛酸苯腙,产物在324 nm处具有特征吸收峰,通过吸光值的变化即可表征乙醇酸氧化酶的活性。
  • 检测方法: 乙醇酸比色法
  • 检测波长: 324 nm
  • 信号响应: 递增型
Notices

①若A1测定大于0.9,建议将粗酶液使用提取液适当稀释后再测定,计算时相应修改;

②反应体系中底物易发生自氧化,设置空白组作为试剂质量检测孔,正常情况下∆A空白应小于0.02,试验前可测定空白组以确定底物状态;

③准确在10 s和130 s处完成读数,以保证实验结果的准确性;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhong Y, Xu J, Zhao X, et al. Balancing Damage via Non-Photochemical Quenching, Phenolic Compounds and Photorespiration in Ulva prolifera Induced by Low-Dose and Short-Term UV-B Radiation[J]. International Journal of Molecular Sciences, 2022, 23(5): 2693.(IF 6.208)

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