①叶绿素对光敏感,研磨和提取等操作尽量避光或者在弱光下进行;
②提取时应浸提至组织残渣完全变白,否则会因提取不充分而影响测定结果;
③提取液冲洗研钵时应冲洗至所有的绿色物质转移至试管/离心管中;
④若测定吸光值超过1.0,建议将浸提液使用提取液适当稀释后再进行测定,计算时相应调整(按实际稀释倍数调整计算公式中D);若测定吸光值小于0.05时,可适当减少提取过程中提取液的用量后再进行测定,计算时相应调整(按实际提取后总体积调整计算公式中V提);
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