

①为避免色素见光分解,操作时应尽量避光,研磨或匀浆时应尽量缩短时间;
②若不确定组织中有无叶绿素影响,可取样本浸提液采用分光光度计在波长400-700 nm下进行扫描,640-670 nm之间有吸收峰则表明存在叶绿体;
③若测定吸光值超过1.0,建议将浸提液使用提取液适当稀释后再进行测定,计算时相应调整(按实际稀释倍数调整计算公式中D);若测定吸光值小于0.05时,可适当减少提取过程中提取液的用量后再进行测定,计算时相应调整(按实际提取后总体积调整计算公式中V提);
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