Plant Carotenoids Content Assay Kit
植物类胡萝卜素含量检测试剂盒
货号:AKPL004M
规格: 110T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Plant Carotenoids Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96 孔板
  • 预计测定时间:6 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    丙酮(C3H6O,MW=58.08,CAS: 67-64-1)
Detection Principle
类胡萝卜素在440 ± 10 nm处具有特殊吸收峰,样本经溶剂提取后在此波长下可检测其含量。含叶绿体的组织样本,需排除叶绿素a和叶绿素b对类胡萝卜素的干扰,根据经验公式计算出叶绿素a和叶绿素b的含量,再进一步得出类胡萝卜素的含量;不含叶绿素的组织可以直接根据类胡萝卜素的经验消光系数进行计算。
  • 检测方法: 萃取比色法
  • 检测波长: 440 nm
  • 信号响应: 递增型
Notices

①为避免色素见光分解,操作时应尽量避光,研磨或匀浆时应尽量缩短时间;

②若不确定组织中有无叶绿素影响,可取样本浸提液采用分光光度计在波长400-700 nm下进行扫描,640-670 nm之间有吸收峰则表明存在叶绿体;

③若测定吸光值超过1.0,建议将浸提液使用提取液适当稀释后再进行测定,计算时相应调整(按实际稀释倍数调整计算公式中D);若测定吸光值小于0.05时,可适当减少提取过程中提取液的用量后再进行测定,计算时相应调整(按实际提取后总体积调整计算公式中V提);

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Wang B, Wang Y, Deng Y, et al. Synthesis of betanin by expression of the core betalain biosynthetic pathway in carrot (Daucus carota L.)[J]. Horticultural Plant Journal, 2023.(IF 5.7)

[2] Xing S, Zhu H, Zhou Y, et al. A cytochrome P450 superfamily gene, IbCYP82D47, increases carotenoid contents in transgenic sweet potato[J]. Plant Science, 2022, 318: 111233.(IF 5.363)

[3] Zhou M, Hu S, Wang S, et al. Sweet potato yield and quality characteristics affected by different late‐season irrigation levels[J]. Journal of the Science of Food and Agriculture, 2024. (IF 4.1)

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