Soli Nitrite (S-NiR) Reductase Activity Assay Kit
土壤亚硝酸还原酶活性检测试剂盒
货号:AKEN006C
规格: 70T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
Soli Nitrite (S-NiR) Reductase Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:4 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
亚硝酸还原酶能够将NO2-还原为NO,使样品中参与重氮化反应生成紫红色化合物的NO2-减少,重氮化反应产物在540 nm处具有特征吸收峰,通过吸光值变化即可表征土壤亚硝酸还原酶的活性。
  • 检测方法: 萘胺比色法
  • 检测波长: 540 nm
  • 信号响应: 递减型
Refreence Information
  • 标准物质: Sodium Nitrite
  • 参考标准: y=4.7682x+0.0198 (R2=0.9992)
  • 标准线性范围: 0.0125-0.3 μmol/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若测定吸光值超出标准线性吸光值范围:高于最高值建议适当增加样本量或将离心上清液适当稀释后再进行测定,低于最低值建议适当减少样本量后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Ma X, Li Y, Niu L, et al. Microbial community structure and denitrification responses to cascade low-head dams and their contribution to eutrophication in urban rivers[J]. Environmental Research, 2023, 221: 115242.(IF 8.3)

[2] Yu Y, Huang J, Tong Z, et al. Response of microbial community diversity and the abundance of nitrogen-cycling genes to Bacillus subtilis application in mulberry field soil[J]. Soil Research, 2024, 62(2). (IF 1.6)

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