Soil β-Xylosidase (S-β-XYS) Activity Assay Kit
土壤β-木糖苷酶活性检测试剂盒
货号:AKEN015M
规格: 110T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Soil β-Xylosidase (S-β-XYS) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96 孔板
  • 预计测定时间:3 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    甲苯(C7H8,MW=96.14,CAS:108-88-3)
Detection Principle
土壤β-木糖苷酶能够催化对硝基苯酚-β-D-木糖苷生成对-硝基苯酚,产物在400 nm处具有特征吸收峰,通过吸光值增加速率即可表征土壤β-木糖苷酶的活性。
  • 检测方法: 对-硝基苯酚法
  • 检测波长: 400 nm
  • 信号响应: 递增型
Notices
①若ΔA测定大于1.0,建议将第一次离心上清液适当稀释后重新加入试剂四显色后再进行测定,若ΔA测定小于0.02时,建议适当延长50℃反应时间后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhou J, Miao Y, Guo L, et al. Evaluating the Enzyme Activities and Soil Physicochemical Properties of Four Typical Halophytic Communities in Saline-Sodic Soil[J]. Agronomy, 2024, 14(1): 141.(IF 3.7)

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