①若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本适当稀释后再进行测定,低于最低值建议适当增加样本量或缩小待测样本稀释倍数后再进行测定,计算时相应修改;
②浓硫酸具有强腐蚀性,操作时需特别注意:加入浓硫酸时,建议将枪头置于液面以下并缓慢加入,以防止液面沸腾烫伤及样本碳化,95℃水浴结束后需冷却至室温再打开离心管盖,以防液体飞溅;
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