Plant Soluble Sugar Content Assay Kit
植物可溶性糖含量检测试剂盒
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Product Information
Plant Soluble Sugar Content Assay Kit
4℃ Wet Ice Transportation
- 检测样本量：100 Samples
- 主要检测设备及配套：酶标仪/96 孔板
- 预计测定时间：2 h (100 Samples)
- 试剂储存条件：按照标签要求储存
-
需自备试剂:浓硫酸（H2SO4，MW=98.078，CAS: 7664-93-9）
Detection Principle
糖类物质在硫酸作用下，脱水生成糠醛或羟甲基糠醛，进一步与蒽酮脱水缩合生成蓝绿色衍生物，产物在620 nm处具有特征吸收峰，通过吸光值变化即可定量检测植物可溶性糖的含量。
- 检测方法： 蒽酮比色法
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检测波长： 620 nm
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信号响应： 递增型
Refreence Information
- 标准物质： Glucose
- 参考标准： y=3.4351x+0.004(R2=0.9995)
- 标准线性范围： 0.00625-0.3 mg/mL
- 检测限： 0.003 mg/mL
- 注：不同仪器及比色材质会对结果产生影响，以实际测定值为准。
Notices
①若测定吸光值超出标准吸光值线性范围：高于最高值建议将待测样本适当稀释后再进行测定，低于最低值建议适当增加样本量或缩小待测样本稀释倍数后再进行测定，计算时相应修改；
②浓硫酸具有强腐蚀性，操作时需特别注意：加入浓硫酸时，建议将枪头置于液面以下并缓慢加入，以防止液面沸腾烫伤及样本碳化，95℃水浴结束后需冷却至室温再打开离心管盖，以防液体飞溅；
注： 为保证结果准确且避免试剂损失，测定前请仔细阅读说明书（以实际收到说明书为准），确认试剂储存和准备是否充分，操作步骤是否清楚，且务必取2-3个预期差异交的样本进行预测定，过程中问题请您及时与工作人员联系。
Product Citation
[1] Dong Z, Liu Z, Xu Y, et al. Potential for the development of Taraxacum mongolicum aqueous extract as a phytogenic feed additive for poultry[J]. Frontiers in Immunology, 2024, 15: 1354040. (IF 7.3)
[2] Liu Z, Hu Y, Du A, et al. Cell Wall Matrix Polysaccharides Contribute to Salt–Alkali Tolerance in Rice[J]. International Journal of Molecular Sciences, 2022, 23(23): 15019.(IF 6.208)
[3] Liu N, Jacquemyn H, Liu Q, et al. Effects of a dark septate fungal endophyte on the growth and physiological response of seedlings to drought in an epiphytic orchid[J]. Frontiers in Microbiology, 2022, 13: 961172.(IF 6.064)
[4] Wang B, Wang Y, Deng Y, et al. Synthesis of betanin by expression of the core betalain biosynthetic pathway in carrot (Daucus carota L.)[J]. Horticultural Plant Journal, 2023.(IF 5.7)
[5] Li J, Liu X, Ahmad N, et al. CePP2C19 confers tolerance to drought by regulating the ABA sensitivity in Cyperus esculentus[J]. BMC Plant Biology, 2023, 23(1): 524.(IF 5.3)
[6] Li Z, Li X, He F. Non-structural carbohydrates contributed to cold tolerance and regeneration of Medicago sativa L[J]. Planta, 2023, 257(6): 116.(IF 4.3)
[7] Li Z, Li X, He F. Drip Irrigation Depth Alters Root Morphology and Architecture and Cold Resistance of Alfalfa[J]. Agronomy, 2022, 12(9): 2192.(IF 3.949)
[8] Liu T, Fu Y, Li G, et al. Transcriptomic and Physiological Responses of Qingye Ramie to Drought Stress[J]. Agronomy, 2024, 14(2): 301.(IF 3.7)
[9] Wang F H, Zhang C, Wang C L, et al. Estimating the role of maize Y-EPSPS gene in glyphosate resistance in Arabidopsis transgenic lines[J]. Plant Growth Regulation, 2024: 1-13. (IF 3.5)
[10] Hou S, Liu Z, Li Y, et al. Exogenous salicylic acid enhanced resistance of Foxtail Millet (Setaria italica) to Sclerospora graminicola[J]. Plant Growth Regulation, 2023, 99(1): 35-44.(IF 3.242)
[11] Cheng H, Chang S, Shi X, et al. Molecular Mechanisms of the Effects of Sodium Selenite on the Growth, Nutritional Quality, and Species of Organic Selenium in Dandelions[J]. Horticulturae, 2024, 10(3): 209. (IF 3.1)
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Calculator
The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
This equation is commonly abbreviated as: C1V1 = C2V2
Sample(S):可测定的样品数量；
★根据此数量计算需要的试剂盒个数。
Size
120T/100S
Test(T):总反应次数；
★每个试剂盒均预留了至少5个（分光光度法）和10个（微板法）样本预实验的试剂用量；
★若需要制作标准曲线则至少能够满足2次制作标准曲线的试剂用量（15个）。
