Plant Dehydrogenase (PDHA) Activity Assay Kit
植物脱氢酶活性检测试剂盒
货号:AKPL010M-50S
规格: 110T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Plant Dehydrogenase (PDHA) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    乙酸乙酯(Ethyl Acetate,C4H8O2,MW = 88.11,CAS: 141-78-6)
Detection Principle
氢受体2,3,5-氯化三苯基四氮唑(2,3,5-Triphenyl Tetrazolium Chloride,TTC)在细胞呼吸过程中受氢,生成红色三苯基甲臜(Triphenyl Formazone,TF),产物在485 nm处具有特征吸收峰,通过吸光值的变化即可表征植物脱氢酶的活性。
  • 检测方法: TTC法
  • 检测波长: 485 nm
  • 信号响应: 递增型
Notices

①反应完成后立即冰浴以终止反应,并将残留的反应液完全去除;

②乙酸乙酯易挥发,应在通风处中进行操作,并做好防护措施;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Li X, Liang H, Wu Z, et al. Applying a microbial electrolysis cell to promote imidacloprid removal in the adsorption biological coupling system at low temperature via accelerating coke regeneration and bioaugmentation[J]. Chemical Engineering Journal, 2023, 477: 147173.(IF 15.1)

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