Plant Total Phenol Content Assay Kit
植物总酚含量检测试剂盒
货号:AKPL016C-50S
规格: 120T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
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Product Information
Plant Total Phenol Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:2 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
酚类物质在碱性条件下能够将钨钼酸还原生成蓝色化合物,产物在760 nm处具有特征吸收峰,通过吸光值变化即可定量检测总酚的含量。
  • 检测方法: Folin-Ciocalteu
  • 检测波长: 760 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Gallic Acid
  • 参考标准: y=5.4584x+0.0157 (R2=0.9992)
  • 标准线性范围: 0.0125-0.2 mg/mL
  • 检测限: 0.002 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①若A测定或∆A测定超出标准吸光值线性范围:高于最高值建议将待测样本使用提取液适当稀释后再进行测定;低于最低值建议适当增加样本量重新提取后再进行测定,计算时相应修改;

②提取液中含有蛋白沉淀组分,样本蛋白浓度测定需使用PBS单独提取后再进行测定;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Geng L, Zhang Q, Li Q, et al. Fucoidan from the cell wall of Silvetia siliquosa with immunomodulatory effect on RAW 264.7 cells[J]. Carbohydrate Polymers, 2024: 121883. (IF 11.2)

[2] Zhang Y, Tian X, Huang P, et al. Biochemical and transcriptomic responses of buckwheat to polyethylene microplastics[J]. Science of The Total Environment, 2023, 899: 165587.(IF 9.8)

[3] Wang J, Li L, Wang Z, et al. Integrative analysis of the metabolome and transcriptome reveals the molecular regulatory mechanism of isoflavonoid biosynthesis in Ormosia henryi Prain[J]. International Journal of Biological Macromolecules, 2023, 246: 125601.(IF 8.2)

[4] Cui M, Li X, Geng L, et al. Comparative study of the immunomodulatory effects of different fucoidans from Saccharina japonica mediated by scavenger receptors on RAW 264.7 macrophages[J]. International Journal of Biological Macromolecules, 2022, 215: 253-261.(IF 8.025)

[5] Dong Z, Liu Z, Xu Y, et al. Potential for the development of Taraxacum mongolicum aqueous extract as a phytogenic feed additive for poultry[J]. Frontiers in Immunology, 2024, 15: 1354040. (IF 7.3)

[6] Liu X, Fan H M, Liu D H, et al. Transcriptome and metabolome analyses provide insights into the watercore disorder on “akibae” pear fruit[J]. International Journal Of Molecular Sciences, 2021, 22(9): 4911.(IF 5.924)

[7] Wang B, Wang Y, Deng Y, et al. Synthesis of betanin by expression of the core betalain biosynthetic pathway in carrot (Daucus carota L.)[J]. Horticultural Plant Journal, 2023.(IF 5.7)

[8] Wei S, Geng L, Yu H, et al. Isolation, Characterization, and Anti-Idiopathic Pulmonary Fibrosis Activity of a Fucoidan from Costaria costata[J]. Molecules, 2023, 28(11): 4343.(IF 4.6)

[9] He Q, Jiang Y, Huang C, et al. Molecular mechanism of delayed development by interfering RNA targeting the phenylalanine ammonia lyase gene (pal1) in Pleurotus ostreatus[J]. Journal of Integrative Agriculture, 2024. (IF 4.6)

[10] Cui W, Liu J, Bai Q, et al. Rapid Reduction of Phytotoxicity in Green Waste for Use as Peat Substitute: Optimization of Ammonium Incubation Process[J]. Plants, 2024, 13(17): 2360. (IF 4.0)

[11] Li L, Wu S, Wang S, et al. Molecular Mechanism of Exogenous Selenium Affecting the Nutritional Quality, Species and Content of Organic Selenium in Mustard[J]. Agronomy, 2023, 13(5): 1425.(IF 3.7)

[12] Tian Y, Yang Z, Song W, et al. Biofumigation by Mustard Plants as an Application for Controlling Postharvest Gray Mold in Apple Fruits[J]. Agronomy, 2023, 13(6): 1490.(IF 3.7)

[13] Li X, Xiu D, Huang J, et al. Nutshell Physicochemical Characteristics of Different Hazel Cultivars and Their Defensive Activity toward Curculio nucum (Coleoptera: Curculionidae)[J]. Forests, 2023, 14(2): 319.(IF 2.9)

[14] Chen Z, Wang A, Qin W, et al. Study on the microbial inactivation of whole hulless barley flour using a continuous instant pressure drop sterilizer[J]. Journal of Food Process Engineering, 2022, 45(12): e14169.(IF 2.889)

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