Tannin Content Assay Kit
单宁含量检测试剂盒
货号:AKPL019M
规格: 120T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Tannin Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96 孔UV板
  • 预计测定时间:5 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
单宁在275 nm处具有特征吸收峰,活性炭能够特异吸附单宁,通过测定275 nm处吸光值变化即可定量检测单宁的含量。
  • 检测方法: 活性炭吸附法
  • 检测波长: 275 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Tannin
  • 参考标准: y=0.0431x+0.0037 (R2=1)
  • 标准线性范围: 1.25-30 nmol/mL
  • 检测限: 1.0 nmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①若测定吸光值超出标准线性吸光值范围:高于最高值建议将待测样本使用提取液适当稀释后再进行测定,低于最低值建议适当增加样本量或调整提取液加入量重新提取后再进行测定,计算时相应修改;

②样本蛋白浓度测定需使用PBS单独提取后再进行测定;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhang D, Wang Q, Li Z, et al. Changing the polyphenol composition and enhancing the enzyme activity of sorghum grain by solid‐state fermentation with different microbial strains[J]. Journal of the Science of Food and Agriculture, 2024. (IF 4.1)

[2] Li X, Xiu D, Huang J, et al. Nutshell Physicochemical Characteristics of Different Hazel Cultivars and Their Defensive Activity toward Curculio nucum (Coleoptera: Curculionidae)[J]. Forests, 2023, 14(2): 319.(IF 2.9)

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