

①A1应控制在0.1-1范围内,可提高检测灵敏度:若A1大于1,建议适当减少反应体系中待测样本的添加量,保持总体积1 mL不变(如50 μL待测样本和950 μL试剂一/试剂二);若A1小于0.1,建议适当增加反应体系中待测样本的添加量,保持总体积1 mL不变(如500 μL待测样本和500 μL试剂一/试剂二),计算时相应修改V样的体积;
②提取液会使蛋白变性,若使用蛋白浓度计算需用PBS单独提取后再进行蛋白浓度测定;[1] Li Y, Song Z, Zhan X, et al. Chromosome-level genome assembly assisting for dissecting mechanism of anthocyanin regulation in kiwifruit (Actinidia arguta)[J]. Molecular Horticulture, 2025, 5(1): 18. (IF 10.6)
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