Soil Acid Protease (S-ACPT) Activity Assay Kit
土壤酸性蛋白酶活性检测试剂盒
货号:AKEN031C
规格: 70T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
Soil Acid Protease (S-ACPT) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:25 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
土壤酸性蛋白酶能够在酸性条件下催化酪蛋白水解产生酪氨酸,酪氨酸在碱性条件下能够还原磷钼酸生成钨蓝,产物在680 nm处具有特征吸收峰,通过吸光值变化即可表征土壤酸性蛋白酶活性。
  • 检测方法: Folin法
  • 检测波长: 680 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Tyrosine
  • 参考标准: y=2.3784x+0.016(R2=0.9995)
  • 标准线性范围: 0.025-0.6 μmol/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若A测定超出标准吸光值线性范围:高于最高值建议将离心后上清液适当稀释后再进行测定;低于最低值建议适当增加样本量后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhang S, Li Q, Huang Z, et al. Exploring community succession and metabolic changes in corn gluten meal-bran mixed wastes during fermentation[J]. Journal of Environmental Management, 2024, 366: 121684. (IF 8.0)

[2] Zhang S, Huang Z, Li Q, et al. Two-stage solid-state fermentation to increase the nutrient value of corn processing waste and explore its efficacy as a feed protein source[J]. Food Chemistry: X, 2024, 23: 101656. (IF 6.5)

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