Soil Alkaline Protease (S-ALPT) Activity Assay Kit
土壤碱性蛋白酶活性检测试剂盒
货号:AKEN033M
规格: 120T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Soil Alkaline Protease (S-ALPT) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96 孔板
  • 预计测定时间:25 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
土壤碱性蛋白酶能够在碱性条件下催化酪蛋白水解产生酪氨酸,酪氨酸在碱性条件下能够还原磷钼酸生成钨蓝,产物在680 nm处具有特征吸收峰,通过吸光值变化即可表征土壤碱性蛋白酶活性。
  • 检测方法: Folin法
  • 检测波长: 680 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Tyrosine
  • 参考标准: y=1.1409x+0.0136(R2=0.9996)
  • 标准线性范围: 0.05-1.0 μmol/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若A测定超出标准吸光值线性范围:高于最高值建议将离心后上清液适当稀释后再进行测定;低于最低值建议适当增加样本量后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Yu Y, Huang J, Tong Z, et al. Response of microbial community diversity and the abundance of nitrogen-cycling genes to Bacillus subtilis application in mulberry field soil[J]. Soil Research, 2024, 62(2). (IF 1.6)

[2] Yu S U N, Li J I, CHANG J, et al. Compositional and functional succession of soil bacterial communities during long-term rice cultivation on saline-alkali soils: Insights derived from a new perspective of the core bacterial taxa[J]. Pedosphere, 2024. (IF 5.2)

[3] Tao A, Chen Z, Wang K, et al. Effects of phosphorus sources on the transformation of phosphorus forms, microbial community, and functional genes in up-flow anaerobic sludge bed reactor[J]. Biochemical Engineering Journal, 2024: 109382. (IF 3.7)

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