Starch Content Assay Kit
淀粉含量检测试剂盒
货号:AKSU015M
规格: 120T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Starch Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96 孔板
  • 预计测定时间:4 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    浓硫酸(H2SO4,MW = 98.08,CAS: 7664-93-9)
Detection Principle
使用洗脱液将样品中可溶性糖与淀粉分开,淀粉经酸水解为葡萄糖,进一步与蒽酮反应生成蓝绿色糠醛衍生物,产物在620 nm处具有特征吸收峰,通过吸光值变化即可定量检测淀粉的含量。
  • 检测方法: 蒽酮比色法
  • 检测波长: 620 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Glucose
  • 参考标准: y=3.7681x+0.025 (R2=0.9989)
  • 标准线性范围: 0.01-0.25 mg/mL
  • 检测限: 0.008 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①若A测定或∆A测定超出标准线性吸光值范围:高于最高值建议将待测样本使用蒸馏水适当稀释后再进行测定;低于最低值建议制备更高浓度样本后再进行测定,计算时相应修改;

②浓硫酸具有强腐蚀性,请做好防护措施并谨慎操作;

③待测样本稀释倍数可参照公式计算

最大稀释倍数(DMax)=74"×W×A"

最小稀释倍数(DMin)=3"×W×A"

注释:W:样品质量,g;A:预估淀粉含量,mg/g;例:预估淀粉含量为400 mg/g(40%),称取0.05 g样本进行淀粉提取,DMin=60,DMax=1480,即待测样本稀释倍数可选择60-1400倍。

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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[2] Tian X, Hu Y, Gao Y, et al. Effects of Aspergillus flavus infection on multi-scale structures and physicochemical properties of maize starch during storage[J]. Carbohydrate Polymers, 2024: 122322. (IF 11.2)

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[4] He Z, Zeng J, Hu J, et al. Effects of cooking methods on the physical properties and in vitro digestibility of starch isolated from Chinese yam[J]. International Journal of Biological Macromolecules, 2024: 131597. (IF 8.2)

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[6] Wu S, Zhang Y, Chen B, et al. Combined treatment of rice bran by solid-state fermentation and extrusion: Effect of processing sequence and microbial strains[J]. Food Chemistry: X, 2024: 101549. (IF 6.5)

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[12] Hou S, Han J, Men Y, et al. Analysis of genotype-by-environment effects on starch content in 281 Tartary buckwheat varieties and evaluation of the physicochemical properties of two elite varieties[J]. LWT, 2024: 115866. (IF 6.0)

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[18] Wan X, Yao G, Wang K, et al. Transcriptomic Analysis of the Response of the Toxic Dinoflagellate Prorocentrum lima to Phosphorous Limitation[J]. Microorganisms, 2023, 11(9): 2216.(IF 4.5)

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