①反应完成后应在20 min内完成吸光值测定,以防止褪色,不建议一次性测定较多样本;
②若A测定超出标准线性吸光范围:高于最高值建议将待测样本使用试剂四适当稀释后再进行测定,低于最低值建议适当增加样本量或减少试剂四的体积后再进行测定,计算时相应修改;
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