Amylopectin Content Assay Kit
支链淀粉含量检测试剂盒
货号:AKSU017M
规格: 120T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Amylopectin Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:3 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    乙醚(C4H10O,MW=74.12,CAS: 60-29-7);
    无水乙醇(C2H6O,MW=46.07,CAS: 64-17-5)
Detection Principle
支链淀粉与碘能够形成红紫色络合物,通过测定530 nm和755 nm处吸光值的变化即可定量检测支链淀粉的含量。
  • 检测方法: 碘比色法
  • 检测波长: 530 nm & 755 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Amylopectin
  • 参考标准: y=0.4068x+0.0054 (R2=0.9996)
  • 标准线性范围: 0.05-1.0 mg/mL
  • 检测限: 0.01 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①反应完成后应在20 min内完成吸光值测定,以防止褪色,不建议一次性测定较多样本;

②若A测定超出标准线性吸光范围:高于最高值建议将待测样本使用试剂四适当稀释后再进行测定,低于最低值建议适当增加样本量或减少试剂四的体积后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Tian X, Hu Y, Gao Y, et al. Effects of Aspergillus flavus infection on multi-scale structures and physicochemical properties of maize starch during storage[J]. Carbohydrate Polymers, 2024: 122322. (IF 11.2)

[2] Wei F, Ma N, Haseeb H A, et al. Insights into structural and physicochemical properties of maize starch after Fusarium verticillioides infection[J]. Journal of Food Composition and Analysis, 2022, 114: 104819.(IF 4.52)

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