①若测定吸光值超出标准线性吸光值范围,可以增加样本量或适当稀释样本后再进行测定,计算时相应修改;
②建议使用新鲜样本进行检测,试剂二具有腐蚀性,操作时注意做好防护措施;
③组织颜色对检测结果无影响,若液体样本含有颜色时(550 nm处具有吸收峰),则需添加对照管,即将试剂一和显色液使用相同体积蒸馏水代替。测定A对照,计算∆A测定=A测定-A对照即可;
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