Nitric Oxide (NO) Content Assay Kit
一氧化氮含量检测试剂盒
货号:AKNM005M
规格: 100T/96S
检测设备:酶标仪
可检测样本数:96 Samples
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Product Information
Nitric Oxide (NO) Content Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:96 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:5 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
NO极易氧化生成NO2-和NO3-,通过硝酸还原酶将NO3-还原为NO2-,NO2-在酸性条件下与重氮盐磺酸胺生成重氮化合物,再与萘基乙烯基二胺偶合,产物在550 nm处具有特征吸收峰,通过吸光值的变化即可定量检测NO的含量。
  • 检测方法: 硝酸还原酶法
  • 检测波长: 550 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Sodium Nitrite
  • 参考标准: To Be Updated
  • 标准线性范围: 0.003125-0.2 μmol/mL
  • 检测限: 0.001 μmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①若测定吸光值超出标准线性吸光值范围,可以增加样本量或适当稀释样本后再进行测定,计算时相应修改;

②建议使用新鲜样本进行检测,试剂二具有腐蚀性,操作时注意做好防护措施;

③组织颜色对检测结果无影响,若液体样本含有颜色时(550 nm处具有吸收峰),则需添加对照管,即将试剂一和显色液使用相同体积蒸馏水代替。测定A对照,计算∆A测定=A测定-A对照即可;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhou L, Zhao B, Zhuang W Q. Double-edged sword effects of dissimilatory nitrate reduction to ammonium (DNRA) bacteria on anammox bacteria performance in an MBR reactor[J]. Water Research, 2023, 233: 119754.(IF 12.8)

[2] Zhou L, Zhao B, Lin Y, et al. Identification of dissimilatory nitrate reduction to ammonium (DNRA) and denitrification in the dynamic cake layer of a full-scale anoixc dynamic membrane bioreactor for treating hotel laundry wastewater[J]. Chemosphere, 2022, 307: 136078.(IF 8.943)

[3] Weng S, Li Q, Zhang T, et al. Enhanced Glycosylation Caused by Overexpression of Rv1002c in a Recombinant BCG Promotes Immune Response and Protects against Mycobacterium tuberculosis Infection[J]. Vaccines, 2024, 12(6): 622. (IF 5.2)

[4] Shao W, Li Z, Wang B, et al. Dimethyloxalylglycine Attenuates Steroid-Associated Endothelial Progenitor Cell Impairment and Osteonecrosis of the Femoral Head by Regulating the HIF-1α Signaling Pathway[J]. Biomedicines, 2023, 11(4): 992.(IF 4.7)

[5] Zhang H, Zu Q, Zhang J, et al. Soluble Dietary Fiber of Hawthorn Relieves Constipation Induced by Loperamide Hydrochloride by Improving Intestinal Flora and Inflammation, Thereby Regulating the Aquaporin Ion Pathway in Mice[J]. Foods, 2024, 13(14): 2220. (IF 4.7)

[6] Du P, Song J, Qiu H, et al. Polyphenols Extracted from Shanxi-Aged Vinegar Inhibit Inflammation in LPS-Induced RAW264. 7 Macrophages and ICR Mice via the Suppression of MAPK/NF-κB Pathway Activation[J]. Molecules, 2021, 26(9): 2745.(IF 4.412)

[7] Xu W, Xu S, Feng R, et al. Yunpi Tongbian Fang alleviates slow transit constipation induced by loperamide by regulating intestinal microbiota and short-chain fatty acids in rats[J]. Cellular and Molecular Biology, 2023, 69(10): 23-29.(IF 1.6)

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