①测定过程中粗酶液和HK工作液需置于冰上保存以免失活;
②准确在相应时间点完成测定,若使用96孔UV板进行测定,建议使用多道移液器且分批进行测定,以保证组间反应时间统一;
③若ΔA>0.5建议将粗酶液使用提取液适当稀释或缩短反应时间至2 min后再进行测定,使ΔA<0.5以提高检测灵敏度,计算时相应修改;
[1] Zhang Y, Gao Y, Wang J, et al. Bioenergetic Metabolism Modulatory Peptide Hydrogel for Cardiac Protection and Repair After Myocardial Infarction[J]. Advanced Functional Materials, 2024: 2312772.(IF 19)
[2] Li Q, Song H, Li S, et al. Macrophage metabolism reprogramming EGCG-Cu coordination capsules delivered in polyzwitterionic hydrogel for burn wound healing and regeneration[J]. Bioactive Materials, 2023, 29: 251-264.(IF 18.9)
[3] Huang K L, Tian J, Wang H, et al. Fatty acid export protein BnFAX6 functions in lipid synthesis and axillary bud growth in Brassica napus[J]. Plant Physiology, 2021, 186(4): 2064-2077.(IF 8.34)
[4] Tang X, Su Y, Ye H, et al. Glucose-potentiated amikacin killing of cefoperazone/sulbactam resistant Pseudomonas aeruginosa[J]. Frontiers in Microbiology, 2022, 12: 800442.(IF 6.064)
[5] Chen Y, Yang K, Dai Z, et al. Repressed central carbon metabolism and its effect on related metabolic pathways in cefoperazone/sulbactam-resistant Pseudomonas aeruginosa[J]. Frontiers in Microbiology, 2022, 13: 847634.(IF 6.064)
[6] Wei Y, Wang T, Nie X, et al. 1, 25-Dihydroxyvitamin D3 Provides Benefits in Vitiligo Based on Modulation of CD8+ T Cell Glycolysis and Function[J]. Nutrients, 2023, 15(21): 4697.(IF 5.9)
[7] Guo L, Zong Y, Yang W, et al. DCBLD2 deletion increases hyperglycemia and induces vascular remodeling by inhibiting insulin receptor recycling in endothelial cells[J]. The FEBS Journal, 2024. (IF 5.5)
[8] Lin J, Lan Y, Xiang D, et al. IL-33 promotes pancreatic β-cell survival and insulin secretion under diabetogenic conditions through PPARγ[J]. European Journal of Pharmacology, 2023, 959: 176059.(IF 5)
[9] Dai T, Yang Y, Zhang J, et al. GCK exonic mutations induce abnormal biochemical activities and result in GCK-MODY[J]. Frontiers in Genetics, 2023, 14: 1120153.(IF 3.7)