①测定过程中试剂一、试剂二和粗酶液须冰上放置以免失活;
②准确在相应时间点完成吸光值的测定,以保证实验结果的准确性;若使用96孔UV板应使用多道移液器且分批进行检测,以确保组间反应时间一致;
③若∆A≥0.5(微量石英比色皿)或0.3(96孔UV板)建议将粗酶液使用提取液适当稀释后再进行测定,或适当缩短反应时间使∆A<0.5或0.3,以提高检测的灵敏度,计算时相应修改;
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