Phosphofructokinase (PFK) Activity Assay Kit
磷酸果糖激酶活性检测试剂盒
货号:AKSU062M
规格: 110T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Phosphofructokinase (PFK) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔UV板
  • 预计测定时间:5 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
磷酸果糖激酶催化果糖-6-磷酸和ATP生成果糖二磷酸和ADP,丙酮酸激酶和乳酸脱氢酶依次催化NADH氧化生成NAD+,NADH在340 nm处具有特征吸收峰,通过吸光值的下降速率即可表征磷酸果糖激酶的活性。
  • 检测方法: NADH速率法
  • 检测波长: 340 nm
  • 信号响应: 递减型
Notices

①测定过程中试剂一、试剂二和粗酶液须冰上放置以免失活;

②准确在相应时间点完成吸光值的测定,以保证实验结果的准确性;若使用96孔UV板应使用多道移液器且分批进行检测,以确保组间反应时间一致;

③若∆A≥0.5(微量石英比色皿)或0.3(96孔UV板)建议将粗酶液使用提取液适当稀释后再进行测定,或适当缩短反应时间使∆A<0.5或0.3,以提高检测的灵敏度,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhang Y, Gao Y, Wang J, et al. Bioenergetic Metabolism Modulatory Peptide Hydrogel for Cardiac Protection and Repair After Myocardial Infarction[J]. Advanced Functional Materials, 2024: 2312772.(IF 19)

[2] Li Q, Song H, Li S, et al. Macrophage metabolism reprogramming EGCG-Cu coordination capsules delivered in polyzwitterionic hydrogel for burn wound healing and regeneration[J]. Bioactive Materials, 2023, 29: 251-264.(IF 18.9)

[3] Wu J, Tan Z, Li H, et al. Melatonin reduces proliferation and promotes apoptosis of bladder cancer cells by suppressing O‐GlcNAcylation of cyclin‐dependent‐like kinase 5[J]. Journal of Pineal Research, 2021, 71(3): e12765.(IF 13.007)

[4] Huang K L, Tian J, Wang H, et al. Fatty acid export protein BnFAX6 functions in lipid synthesis and axillary bud growth in Brassica napus[J]. Plant Physiology, 2021, 186(4): 2064-2077.(IF 8.34)

[5] Guo L, Zong Y, Yang W, et al. DCBLD2 deletion increases hyperglycemia and induces vascular remodeling by inhibiting insulin receptor recycling in endothelial cells[J]. The FEBS Journal, 2024. (IF 5.5)

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