β-1,3-Glucanase (β-1,3-GA) Activity Assay Kit
β-1,3葡聚糖酶活性检测试剂盒
货号:AKSU038M-100S
规格: 240T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
β-1,3-Glucanase (β-1,3-GA) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:5 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
β-1,3葡聚糖酶能够内切昆布多糖中的β-1,3-葡萄糖苷键产生还原末端,进一步与DNS反应生成棕红色化合物,产物在540 nm处具有特征吸收峰,通过吸光值变化即可表征β-1,3葡聚糖酶的活性。
  • 检测方法: DNS比色法
  • 检测波长: 540 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Glucose
  • 参考标准: y=1.0819x-0.1308 (R2=0.9996)
  • 标准线性范围: 0.2-1.5 mg/mL
  • 检测限: -
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若A测定或ΔA测定超出标准吸光值线性范围:高于最高值建议将粗酶液适当稀释后再进行测定;低于最低值建议适当增加样本量或适当延长37℃反应时间后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Yu M, Fan Y, Li X, et al. LESION MIMIC MUTANT 1 confers basal resistance to Sclerotinia sclerotiorum in rapeseed via a salicylic acid-dependent pathway[J]. Journal of Experimental Botany, 2023, 74(18): 5620-5634.(IF 6.9)

[2] Li G, Mo Y, Lv J, et al. Unraveling verticillium wilt resistance: insight from the integration of transcriptome and metabolome in wild eggplant[J]. Frontiers in Plant Science, 2024, 15: 1378748. (IF 5.6)

[3] Ge M, Cai X, Wang D, et al. Efficacy of Streptomyces murinus JKTJ-3 in Suppression of Pythium Damping-Off of Watermelon[J]. Microorganisms, 2023, 11(6): 1360.(IF 4.5)

[4] Wang W, He L, Zhang Z, et al. Activity of the botanical compound thymol against kiwifruit rot caused by Fusarium tricinctum and the underlying mechanisms[J]. Pest Management Science, 2023.(IF 4.1)

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