Chitinase Activity Assay Kit
几丁质酶活性检测试剂盒
货号:AKSU045C
规格: 50T/24S
检测设备:可见分光光度计
可检测样本数:24 Samples
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Product Information
Chitinase Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:24 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:4 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
几丁质酶能够水解几丁质产生N-乙酰氨基葡萄糖(NAG),N-乙酰氨基葡萄糖与碱共热产生的中间化合物可进一步与对二甲氨基苯甲醛反应产生显色物质,产物在585 nm处具有特征吸收峰,通过吸光值变化即可表征几丁质酶的活性。
  • 检测方法: 对二甲氨基苯甲醛法
  • 检测波长: 585 nm
  • 信号响应: 递增型
Notices
①若测定吸光值超出标准吸光值线性范围:高于最高值建议将粗酶液适当稀释后或适当缩短37℃水浴时间后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Xu L, Zhao J, Xu D, et al. Application of transcriptomic analysis to unveil the toxicity mechanisms of fall armyworm response after exposure to sublethal chlorantraniliprole[J]. Ecotoxicology and Environmental Safety, 2022, 230: 113145.(IF 6.291)

[2] Zhang Y, Zhuang W Y. MAPK cascades mediating biocontrol activity of trichoderma brevicrassum strain TC967[J]. Journal of agricultural and food chemistry, 2022, 70(8): 2762-2775.(IF 5.895)

[3] Li G, Mo Y, Lv J, et al. Unraveling verticillium wilt resistance: insight from the integration of transcriptome and metabolome in wild eggplant[J]. Frontiers in Plant Science, 2024, 15: 1378748. (IF 5.6)

[4] Zhao Y, Li C, Chen X, et al. Overexpression of chitinase PbChia1 from Plasmodiophora brassicae improves broad-spectrum disease resistance of Arabidopsis[J]. Virulence, 2023, 14(1): 2233147.(IF 5.2)

[5] Zeng Z, Zou Y, Lin F C, et al. Roles of CcDFR and CcOMT9 in the cyanidin biosynthesis and development of Cordyceps cicadae[J]. Frontiers in Microbiology, 2024, 15: 1353710. (IF 5.2)

[6] Liu H, Yang X, Mai L, et al. Comparative Proteomic Analysis of Bacillus subtilis and Aspergillus niger in Black Soldier Fly Co-Fermentation[J]. Fermentation, 2022, 8(11): 593.(IF 5.123)

[7] Ge M, Cai X, Wang D, et al. Efficacy of Streptomyces murinus JKTJ-3 in Suppression of Pythium Damping-Off of Watermelon[J]. Microorganisms, 2023, 11(6): 1360.(IF 4.5)

[8] Song X, Duan Y Y, Tan F Q, et al. Comparative transcriptome analysis of salt tolerance of roots in diploid and autotetraploid citrus rootstock (C. junos cv. Ziyang xiangcheng) and identification of salt tolerance-related genes[J]. Scientia Horticulturae, 2023, 317: 112083.(IF 4.3)

[9] Li Y, Li S, Liang Z, et al. RNA-seq analysis of Rhizoctonia solani AG-4HGI strain BJ-1H infected by a new viral strain of Rhizoctonia solani partitivirus 2 reveals a potential mechanism for hypovirulence[J]. Phytopathology®, 2022, 112(6): 1373-1385.(IF 4.01)

[10] Li X, Zou Y, Shrivastava N, et al. A rho-type GTPase activating protein affects the growth and development of Cordyceps cicadae[J]. Archives of Microbiology, 2024, 206(8): 1-16. (IF 2.3)

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