Basic Xylanase (BAX) Activity Assay Kit
碱性木聚糖酶活性检测试剂盒
货号:AKSU049M
规格: 110T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Basic Xylanase (BAX) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
碱性木聚糖酶能够在碱性环境下将木聚糖降解为还原性寡糖和单糖,进一步与3,5-二硝基水杨酸反应生成棕红色氨基化合物,产物在540 nm处具有特征吸收峰,通过吸光值变化即可表征碱性木聚糖酶的活性。
  • 检测方法: DNS比色法
  • 检测波长: 540 nm
  • 信号响应: 递增型
Notices
①若测定吸光值大于1.2,建议将粗酶液使用提取液适当稀释后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Wang S, Long H, Hu X, et al. The co-inoculation of Trichoderma viridis and Bacillus subtilis improved the aerobic composting efficiency and degradation of lignocellulose[J]. Bioresource Technology, 2024, 394: 130285.(IF 11.4)

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