①若测定吸光值超出标准线性吸光值范围:高于最高值建议将待测样本适当稀释后再进行测定;低于最低值建议适当增加样本量后再进行测定,计算时相应修改;
②待测样本不能用于蛋白浓度的测定,若需要使用蛋白浓度计算结果,建议使用PBS重新制备样本后再进行蛋白浓度测定;
③实验过程中弃上清液时应避免吸入沉淀物而影响结果的准确性;
④反应时间对实验结果有较大影响建议精确控制,测定样本较多时建议分批进行测定,以避免组内反应时间不一致对结果造成影响;
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