①待测样本需置于冰上放置,且柠檬酸提取过程需在冰上进行;
②若测定吸光值超出标准线性吸光值范围:高于最高值建议将待测样本适当稀释后再进行测定;低于最低值建议适当增加样本量重新提取后再进行测定,计算时相应修改;
③试剂五为易致癌物质,实验过程中需佩戴手套并做好防护,避免皮肤接触;
④柠檬酸提取液不能用于蛋白含量测定,如需测定蛋白含量,应另取组织进行测定;
⑤若显色30 min后出现明显黑色颗粒,属于正常现象,需将待测样本适当稀释后重新进行测定;
[1] Yao W, Xie Y, Chen R, et al. Promotion of Dentin Biomimetic Mineralization and Bonding Efficacy by Interfacial Control of an Experimental Citric Acid Dental Etching Agent[J]. ACS Applied Materials & Interfaces, 2024. (IF 9.5)
[2] Qiu Y, Su Y, Song J, et al. Carboxymethylation of the polysaccharide from the fermentation broth of Marasmius androsaceus and its antidepressant mechanisms[J]. Food Science and Human Wellness, 2023, 12(6): 2417-2427.(IF 7.0)
[3] Xia Y, Zhang M K, Ye J J, et al. Polymeric Nanoformulations Aimed at Cancer Metabolism Reprogramming with High Specificity to Inhibit Tumor Growth[J]. Biomaterials Science, 2024.(IF 5.8)
[4] Zeng N, Zhang N, Wang D, et al. Regulation of cell differentiation to promote pullulan synthesis in Aureobasidium pullulans NG[J]. Applied Microbiology and Biotechnology, 2023: 1-13.(IF 5.0)