Citric Acid (CA) Content Assay Kit
柠檬酸含量检测试剂盒
货号:AKAC003M
规格: 120T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Citric Acid (CA) Content Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96 孔板
  • 预计测定时间:6 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
柠檬酸能够在酸性条件下将Cr6+还原为Cr3+,并生成柠檬酸铬螯合物,产物在545 nm处具有特征吸收峰,通过吸光值的变化即可定量检测柠檬酸的含量。
  • 检测方法: Cr(Ⅵ)还原法
  • 检测波长: 545 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Citric Acid
  • 参考标准: y=0.0847x+0.0097 (R2=0.9996)
  • 标准线性范围: 0.25-4.0 μmol/mL
  • 检测限: 0.06 μmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①待测样本需置于冰上放置,且柠檬酸提取过程需在冰上进行;

②若测定吸光值超出标准线性吸光值范围:高于最高值建议将待测样本适当稀释后再进行测定;低于最低值建议适当增加样本量重新提取后再进行测定,计算时相应修改;

③试剂五为易致癌物质,实验过程中需佩戴手套并做好防护,避免皮肤接触;

④柠檬酸提取液不能用于蛋白含量测定,如需测定蛋白含量,应另取组织进行测定;

⑤若显色30 min后出现明显黑色颗粒,属于正常现象,需将待测样本适当稀释后重新进行测定;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Yao W, Xie Y, Chen R, et al. Promotion of Dentin Biomimetic Mineralization and Bonding Efficacy by Interfacial Control of an Experimental Citric Acid Dental Etching Agent[J]. ACS Applied Materials & Interfaces, 2024. (IF 9.5)

[2] Qiu Y, Su Y, Song J, et al. Carboxymethylation of the polysaccharide from the fermentation broth of Marasmius androsaceus and its antidepressant mechanisms[J]. Food Science and Human Wellness, 2023, 12(6): 2417-2427.(IF 7.0)

[3] Xia Y, Zhang M K, Ye J J, et al. Polymeric Nanoformulations Aimed at Cancer Metabolism Reprogramming with High Specificity to Inhibit Tumor Growth[J]. Biomaterials Science, 2024.(IF 5.8)

[4] Zeng N, Zhang N, Wang D, et al. Regulation of cell differentiation to promote pullulan synthesis in Aureobasidium pullulans NG[J]. Applied Microbiology and Biotechnology, 2023: 1-13.(IF 5.0)

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Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
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