Oxalic Acid (OA) Content Assay Kit
草酸含量检测试剂盒
货号:AKAC004C
规格: 70T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
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Product Information
Oxalic Acid (OA) Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:3 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
Fe3+在酸性条件下能够与磺基水杨酸反应生成紫色络合物,产物在510 nm处具有特征吸收峰,草酸及草酸根能够使Fe3+与磺基水杨酸的紫色络合物颜色变浅,Fe3+与磺基水杨酸络合物的吸光度随着草酸量的增加而降低,通过吸光值变化即可定量检测草酸的含量。
  • 检测方法: 水杨酸铁络合比色法
  • 检测波长: 510 nm
  • 信号响应: 递减型
Notices

①若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

②若测定样本较多时,可根据使用量按照试剂二:试剂三:试剂四=2:15:1的体积比配制为OA检测工作液(现用现配),反应体系为:50 μL待测样本+450 μL OA检测工作液+500 μL蒸馏水,充分混匀后进行后续测定即可;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhang Y, Tian X, Huang P, et al. Biochemical and transcriptomic responses of buckwheat to polyethylene microplastics[J]. Science of The Total Environment, 2023, 899: 165587.(IF 9.8)

[2] Yang Z, Zhao P, Peng W, et al. Cloning, Expression Analysis, and Functional Characterization of Candidate Oxalate Transporter Genes of HbOT1 and HbOT2 from Rubber Tree (Hevea brasiliensis)[J]. Cells, 2022, 11(23): 3793.(IF 7.666)

[3] Yang Z, Zhao P, Luo X, et al. An Oxalate Transporter Gene, AtOT, Enhances Aluminum Tolerance in Arabidopsis thaliana by Regulating Oxalate Efflux[J]. International Journal of Molecular Sciences, 2023, 24(5): 4516.(IF 5.6)

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