①若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;
②若测定样本较多时,可根据使用量按照试剂二:试剂三:试剂四=2:15:1的体积比配制为OA检测工作液(现用现配),反应体系为:50 μL待测样本+450 μL OA检测工作液+500 μL蒸馏水,充分混匀后进行后续测定即可;
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