![](https://www.boxbio.cn/content/xtheme/bjhz/images/preferential.png)
![](https://www.boxbio.cn/content/xtheme/bjhz/images/make.png)
①测定过程中样本和所有试剂在冰上放置,以免变性和失活;
②比色皿中反应液的温度必须保持37℃或25℃,可在烧杯中装入一定量37℃或25℃蒸馏水,将烧杯放入37℃或25℃水浴锅中,在反应过程中把比色皿连同反应液放在此烧杯中;
③准确在10 s和130 s处完成读数,以保证实验结果的准确性;
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