Citrate Synthase (CS) Activity Assay Kit
柠檬酸合酶活性检测试剂盒
货号:AKAC007C-2
规格: 25T/12S
检测设备:可见分光光度计
可检测样本数:12 Samples
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Product Information
Citrate Synthase (CS) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:12 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:1 h (12 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
柠檬酸合酶催化乙酰CoA和草酰乙酸产生柠檬酰辅酶A,进一步水解产生柠檬酸,DTNB转变成黄色的TNB,产物在412 nm处有特征吸光峰,根据吸光值变化即可表征柠檬酸合酶活性。
  • 检测方法: DTNB法
  • 检测波长: 412 nm
  • 信号响应: 递增型
Notices

①测定过程中样本和所有试剂在冰上放置,以免变性和失活;

②比色皿中反应液的温度必须保持37℃或25℃,可在烧杯中装入一定量37℃或25℃蒸馏水,将烧杯放入37℃或25℃水浴锅中,在反应过程中把比色皿连同反应液放在此烧杯中;

③准确在10 s和130 s处完成读数,以保证实验结果的准确性;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Leng J, Lu J, Hai C, et al. Exploring influence mechanism of small-molecule carbon source on heterotrophic nitrification-aerobic denitrification process from carbon metabolism, nitrogen metabolism and electron transport process[J]. Bioresource Technology, 2023, 387: 129681.(IF 11.4)

[2] Sun X, Zeng C, Wang F, et al. Neuromedin S regulates steroidogenesis through maintaining mitochondrial morphology and function via NMUR2 in goat ovarian granulosa cells[J]. International Journal of Molecular Sciences, 2022, 23(21): 13402.(IF 6.208)

[3] Chen X, Song M, Zhao J, et al. Excessive composite pollution carbon sources enhance the bio-fertilizer efficiency of Tetradesmus obliquus: focused on cultivation period[J]. Environmental Science and Pollution Research, 2023: 1-13.(IF 5.8)

[4] Zhang T, Chu Y, Wang Y, et al. Testosterone deficiency worsens mitochondrial dysfunction in APP/PS1 mice[J]. Frontiers in Aging Neuroscience, 2024, 16: 1390915. (IF 4.8)

[5] Hou S, Yang P. BPA biodegradation driven by isolated strain SQ-2 and its metabolism mechanism elucidation[J]. Biochemical Engineering Journal, 2022, 185: 108540.(IF 4.446)

[6] Tian S, Xu Y, Zhong Y, et al. Exploring the Organic Acid Secretion Pathway and Potassium Solubilization Ability of Pantoea vagans ZHS-1 for Enhanced Rice Growth[J]. Plants, 2024, 13(14). (IF 4.0)

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