Ascorbic Acid (AsA) Content Assay Kit
抗坏血酸含量检测试剂盒
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Product Information
Ascorbic Acid (AsA) Content Assay Kit
4℃ Wet Ice Transportation
- 检测样本量：100 Samples
- 主要检测设备及配套：酶标仪/96孔UV板
- 预计测定时间：5 h (100 Samples)
- 试剂储存条件：按照标签要求储存
-
需自备试剂:无
Detection Principle
抗坏血酸氧化酶（Ascorbate Oxidase）可催化抗坏血酸氧化生成脱氧抗坏血酸，抗坏血酸在265 nm处具有特征吸收峰，通过吸光值变化速率即可定量检测抗坏血酸的含量。
- 检测方法： AsA比色法
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检测波长： 265 nm
-
信号响应： 递减型
Refreence Information
- 标准物质： Ascorbic Acid
- 参考标准： y=0.0004x-0.0006（R2=0.9992）
- 标准线性范围： 100-1400 µmol/L
- 检测限： 40 µmol/L
- 注：不同仪器及比色材质会对结果产生影响，以实际测定值为准。
Notices
①若同时测定多个样本，可根据样本量按试剂一：试剂二 = 8:1的体积比配制为检测工作液（现用现配，严禁一次性全部配制储存使用）；96孔UV板或微量石英比色皿中加入20 µL待测样本（或20 µL标准应用液），再加入180 µL检测工作液，充分混匀并立即开始计时；
②准确在30 s和150 s处完成吸光值测定，以保证实验结果的准确性和重复性；若使用96孔UV板进行测定应使用多道移液器，且分次进行检测，以确保组间反应时间一致；
③若A1测定大于1.4，建议将待测样本使用提取液适当稀释后再进行测定，计算时相应修改；
注： 为保证结果准确且避免试剂损失，测定前请仔细阅读说明书（以实际收到说明书为准），确认试剂储存和准备是否充分，操作步骤是否清楚，且务必取2-3个预期差异交的样本进行预测定，过程中问题请您及时与工作人员联系。
Product Citation
[1] Sun W, Wu G, Xu H, et al. Malate-mediated CqMADS68 enhances aluminum tolerance in quinoa seedlings through interaction with CqSTOP6, CqALMT6 and CqWRKY88[J]. Journal of Hazardous Materials, 2022, 439: 129630.(IF 14.224)
[2] Shi L, Zhao X, Zhong K, et al. Physiological mechanism of the response to Cr (VI) in the aerobic denitrifying ectomycorrhizal fungus Pisolithus sp. 1[J]. Journal of Hazardous Materials, 2022, 429: 128318.(IF 14.224)
[3] Jiang T, Ding J, Yuan S, et al. Benchtop Vis-NIR spectroscopy meets machine learning for multi-task analysis in Hongmeiren citrus: Geographical origin identification and antioxidant component quantification[J]. Food Chemistry, 2025: 145007. (IF 9.8)
[4] Xu J, Tao X, Liu X, et al. Wearable eye patch biosensor for noninvasive and simultaneous detection of multiple biomarkers in human tears[J]. Analytical Chemistry, 2022, 94(24): 8659-8667.(IF 8.008)
[5] Chen X, Fan Y, Nan H, et al. G hGME31D identified to regulate AsA activation in response to alkali stress from GME gene family implications in cotton[J]. Environmental Sciences Europe, 2024, 36(1): 191. (IF 6.0)
[6] Dao M, Li J, Wang M, et al. Transcriptomic analysis of Camellia japonica to scale insects infestation and functional characterization of pectin methylesterase gene CjPME28 and polygalacturonase gene CjPG1[J]. Plant Cell Reports, 2025, 44(8): 186. (IF 4.5)
[7] Yin M, Zhang J, Du L, et al. Effects of irrigation and organic fertilizer on pumpkin yield, quality, and water-fertilizer use efficiency in arid northwest China[J]. Frontiers in Plant Science, 2025, 16: 1517761. (IF 4.1)
[8] Zhang W, Yu P, Liu W, et al. Mechanism of sodium nitroprusside regulating ginseng quality[J]. Scientific Reports, 2025, 15(1): 1562. (IF 3.8)
[9] Wang H, Lu T, Yan W, et al. Transcriptome and Metabolome Analyses Reveal Ascorbic Acid Ameliorates Cold Tolerance in Rice Seedling Plants[J]. Agronomy, 2024, 14(4): 659. (IF 3.7)
[10] Zhang W, Liu W, Wang L, et al. Effects of water stress on secondary metabolism of Panax ginseng fresh roots[J]. PloS one, 2024, 19(11): e0312023. (IF 2.9)
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Calculator
The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
This equation is commonly abbreviated as: C1V1 = C2V2
Sample(S):可测定的样品数量；
★根据此数量计算需要的试剂盒个数。
Size
110T/100S
Test(T):总反应次数；
★每个试剂盒均预留了至少5个（分光光度法）和10个（微板法）样本预实验的试剂用量；
★若需要制作标准曲线则至少能够满足2次制作标准曲线的试剂用量（15个）。
