Triglyceride (TG) Content Assay kit
甘油三酯含量检测试剂盒
货号:AKFA003C
规格: 70T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
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Product Information
Triglyceride (TG) Content Assay kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:3 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    正庚烷(C7H16,MW=100.2,CAS: 142-82-5);
    异丙醇(C3H8O,MW=60.01,CAS: 67-63-0)
Detection Principle
甘油三酯经KOH皂化水解生成甘油及脂肪酸,过碘酸氧化甘油生成甲醛,在铵离子存在下甲醛能够与乙酰丙酮缩合生成黄色物质,产物在420 nm处具有特征吸收峰,通过吸光值的变化即可定量检测甘油三酯的含量。
  • 检测方法: 乙酰丙酮法
  • 检测波长: 420 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Triglyceride
  • 参考标准: y=0.8603x+0.0253 (R2=0.9992)
  • 标准线性范围: 0.0625-1.5 mg/mL
  • 检测限: 0.06 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①为确保试验结果重复性,加入试剂二后需剧烈震荡,振荡幅度、时间、反复次数以及等待分层时间均应保持一致,检测体系中65℃水浴后冷却时间应保持一致;

②若测定吸光值超出标准吸光值线性范围:高于最高值建议将TG待测液使用试剂一适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

③检测过程中使用具有易挥发性物质,建议在通风橱中进行测定,并做好防护措施;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Chen L, Li J, Yuan R, et al. Dynamic 3D genome reorganization during development and metabolic stress of the porcine liver[J]. Cell Discovery, 2022, 8(1): 56.(IF 38.079)

[2] Shui K, Wang C, Zhang X, et al. Small-sample learning reveals propionylation in determining global protein homeostasis[J]. Nature Communications, 2023, 14(1): 2813.(IF 16.6)

[3] Lin Y, Liu R, Huang Y, et al. Reactivation of PPARα alleviates myocardial lipid accumulation and cardiac dysfunction by improving fatty acid β-oxidation in Dsg2-deficient arrhythmogenic cardiomyopathy[J]. Acta Pharmaceutica Sinica B, 2023, 13(1): 192-203.(IF 14.903)

[4] Tao T, Shu Q, Zhao Y, et al. Mechanical regulation of lipid and sugar absorption by Piezo1 in enterocytes[J]. Acta Pharmaceutica Sinica B, 2024. (IF 14.5)

[5] Liu B, Ding C, Tang W, et al. Hepatic ROS Mediated Macrophage Activation Is Responsible for Irinotecan Induced Liver Injury[J]. Cells, 2022, 11(23): 3791.(IF 7.666)

[6] Yu J, Sun H, Yang Y, et al. Sesamolin alleviates nonalcoholic fatty liver disease through modulating gut microbiota and metabolites in high-fat and high-fructose diet-fed mice[J]. International Journal of Molecular Sciences, 2022, 23(22): 13853.(IF 6.208)

[7] Zhou S, Ma N, Meng M, et al. Lentinan Ameliorates β-Hydroxybutyrate-Induced Lipid Metabolism Disorder in Bovine Hepatocytes by Upregulating the Expression of Acetyl-coenzyme A Acetyltransferase 2[J]. Journal of Agricultural and Food Chemistry, 2024. (IF 5.7)

[8] Jiang J L, Zhou Y Y, Zhong W W, et al. Uridine diphosphate glucuronosyltransferase 1A1 prevents the progression of liver injury[J]. World Journal of Gastroenterology, 2024, 30(9): 1189. (IF 4.3)

[9] Mo H, Liu Y, Zhang M, et al. The Role of Pancreatic Fatty Acid Synthesis in Islet Morphology and Function after Caloric Restriction or Roux-En-Y Gastric Bypass Surgery in Mice[J]. Genes, 2022, 14(1): 5.(IF 4.141)

[10] Zhang S, Xie J, Luo R, et al. MiR-2b-2-5p regulates lipid metabolism and reproduction by targeting CREB in Bactrocera dorsalis[J]. RNA biology, 2023, 20(1): 164-176.(IF 4.1)

[11] Wang S, Yuan T, Yang H, et al. Effect of complete high-caloric nutrition on the nutritional status and survival rate of amyotrophic lateral sclerosis patients after gastrostomy[J]. American Journal of Translational Research, 2022, 14(11): 7842.(IF 3.94)

[12] Zhou S, Chen M, Meng M, et al. Subclinical ketosis leads to lipid metabolism disorder by down-regulating the expression of Acetyl-CoA acetyltransferase 2 (ACAT2) in dairy cows[J]. Journal of Dairy Science, 2023.(IF 3.5)

[13] Song J, Qiu H, Du P, et al. Polyphenols extracted from Shanxi‐aged vinegar exert hypolipidemic effects on OA‐induced HepG2 cells via the PPARα‐LXRα‐ABCA1 pathway[J]. Journal of food biochemistry, 2022, 46(2): e14029.(IF 3.654)

[14] Peng J, Liang G, Li Y, et al. Identification of a novel FOXO3 agonist that protects against alcohol induced liver injury[J]. Biochemical and Biophysical Research Communications, 2024: 149690. (IF 3.1)

[15] Wang X, Huangfu W, Zhao F. Correlation of ChREBP Gene Methylation with Pathological Characteristics of Type 2 Diabetes Mellitus[J]. Applied Biochemistry and Biotechnology, 2023: 1-12.(IF 3)

[16] Yu M, Zhang L, Sun S, et al. Gliquidone improves retinal injury to relieve diabetic retinopathy via regulation of SIRT1/Notch1 pathway[J]. BMC ophthalmology, 2021, 21(1): 1-11.(IF 2.209)

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