Acetylcholinesterase (AchE) Activity Assay Kit
乙酰胆碱酯酶活性检测试剂盒
货号:AKFA005C
规格: 60T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
Acetylcholinesterase (AchE) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:3 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
乙酰胆碱酯酶能够催化乙酰胆碱水解为胆碱,胆碱与二硫对硝基苯甲酸反应生成5-巯基-硝基苯甲酸(TNB),产物在412 nm处具有特征吸收峰,通过吸光值变化即可表征乙酰胆碱酯酶的活性。
  • 检测方法: DTNB法
  • 检测波长: 412 nm
  • 信号响应: 递增型
Notices

①试剂二配制后有效期较短,为便于试验安排,附赠一瓶作为备用;

②测定过程中粗酶液和试剂二应置于冰上放置,以免变性和失活;

③若A测定大于1.0时,建议将粗酶液适当稀释后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Wu J, Ye W, Feng Y, et al. Aquatic Photolysis of High-Risk Fluorinated Liquid Crystal Monomers: Kinetics, Toxicity Evaluation, and Mechanisms[J]. Water Research, 2024: 121510. (IF 12.8)

[2] Deng S, Wang B, Zhang H, et al. Degradation and enhanced oil recovery potential of Alcanivorax borkumensis through production of bio-enzyme and bio-surfactant[J]. Bioresource Technology, 2024: 130690. (IF 11.4)

[3] Cui Y, Wang Z, Zhang G, et al. Transmission of nanoplastics from Culex quinquefasciatus to Pardosa pseudoannulata and its impact on predators[J]. Science of The Total Environment, 2022, 820: 153331.(IF 10.753)

[4] Xu W, Xu S, Feng R, et al. Yunpi Tongbian Fang alleviates slow transit constipation induced by loperamide by regulating intestinal microbiota and short-chain fatty acids in rats[J]. Cellular and Molecular Biology, 2023, 69(10): 23-29.(IF 1.6)

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