Lipase (LPS) Activity Assay Kit
脂肪酶活性检测试剂盒
货号:AKFA006M
规格: 120T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Lipase (LPS) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:9 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    无水乙醇(C2H6O,MW = 46.07,CAS: 64-17-5);
    甲苯(C7H8,MW = 92.14,CAS: 108-88-3)
Detection Principle
脂肪酶催化油酯水解生成游离脂肪酸,利用铜皂法测定脂肪酸生成速率:脂肪酸与显色剂中铜离子反应生成铜皂蓝色络合物,产物在710 nm处具有特征吸收峰,通过吸光值变化即可表征脂肪酶的活性。
  • 检测方法: 铜皂法
  • 检测波长: 710 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Oleic Acid
  • 参考标准: y=0.0121x+0.0369 (R2=0.9996)
  • 标准线性范围: 3.75-120 µmol/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①实验过程中须远离火源,甲苯具有毒性,建议在通风橱中操作并做好防护措施;

②若测定吸光值超出标准吸光值线性范围:高于最高值建议将粗酶液使用试剂一适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Yu S, Jing Y, Fan Y, et al. Ultrahigh efficient emulsification with drag-reducing liquid gating interfacial behavior[J]. Proceedings of the National Academy of Sciences, 2022, 119(29): e2206462119.(IF 12.779)

[2] Xu L, Zhao J, Xu D, et al. Application of transcriptomic analysis to unveil the toxicity mechanisms of fall armyworm response after exposure to sublethal chlorantraniliprole[J]. Ecotoxicology and Environmental Safety, 2022, 230: 113145.(IF 6.291)

[3] Chen X L, Wu L J, Miao L L, et al. Chronic polystyrene microplastics exposure-induced changes in thick-shell mussel (Mytilus coruscus) metaorganism: A holistic perspective[J]. Ecotoxicology and Environmental Safety, 2024, 284: 116961. (IF 6.2)

[4] Liu X, Xie H, Xu Y, et al. Two halogenated flame retardants and cadmium in the soil-rice system: sorption, root uptake, and translocation[J]. Environmental Science and Pollution Research, 2023, 30(43): 97688-97699.(IF 5.8)

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