Free Fatty Acid (FFA) Content Assay Kit
游离脂肪酸含量检测试剂盒
货号:AKFA008C
规格: 70T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
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Product Information
Free Fatty Acid (FFA) Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    正庚烷(C7H16,MW=100.2,CAS: 142-82-5);
    无水甲醇(CH3OH,MW=32.04,CAS: 67-56-1);
    氯仿(CHCl3,MW=119.38,CAS: 67-66-3);
    无水乙醇(C2H6O,MW=46.07,CAS: 64-17-5)
Detection Principle
游离脂肪酸与铜离子结合可形成脂肪酸铜,铜离子能够与显色液反应生成紫红色络合物,产物在550 nm处具有特征吸收峰,通过吸光值变化即可定量检测游离脂肪酸的含量。
  • 检测方法: 铜皂法
  • 检测波长: 550 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Palmitic Acid
  • 参考标准: y=1.2891x-0.0022 (R2=0.9997)
  • 标准线性范围: 0.05-1.0 µmol/mL
  • 检测限: 0.012 µmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①试剂二配制应尽量晚配,可在操作到加入试剂一时,再配制试剂二;

②必须保证每管的震荡频率及时间一致;

③应在30 min内完成测量,测定完成密封好后再丢弃;

④所用试剂多数为有机溶剂,同一支吸头多次吸取会造成体积不准确,建议取液后更换吸头;

⑤若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Lin Y, Liu R, Huang Y, et al. Reactivation of PPARα alleviates myocardial lipid accumulation and cardiac dysfunction by improving fatty acid β-oxidation in Dsg2-deficient arrhythmogenic cardiomyopathy[J]. Acta Pharmaceutica Sinica B, 2023, 13(1): 192-203.(IF 14.903)

[2] Tao T, Shu Q, Zhao Y, et al. Mechanical regulation of lipid and sugar absorption by Piezo1 in enterocytes[J]. Acta Pharmaceutica Sinica B, 2024. (IF 14.5)

[3] Wang W, Zhong Z, Peng S, et al. “All-in-one” metal polyphenol network nanocapsules integrated microneedle patches for lipophagy fueled ferroptosis-mediated multimodal therapy[J]. Journal of Controlled Release, 2024, 373: 599-616. (IF 10.5)

[4] Jinyang W, Yaxin W, Wentao Z, et al. Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stresses[J]. eLife, 2023, 12.(IF 7.7)

[5] Liu H, Ju A, Dong X, et al. Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet β cells in mice[J]. Journal of Translational Medicine, 2023, 21(1): 89.(IF 7.4)

[6] Luo Z, Li M, Yang Q, et al. Ferulic acid prevents nonalcoholic fatty liver disease by promoting fatty acid oxidation and energy expenditure in c57bl/6 mice fed a high-fat diet[J]. Nutrients, 2022, 14(12): 2530.(IF 6.706)

[7] Ji T, Fang B, Wu F, et al. Diet Change Improves Obesity and Lipid Deposition in High-Fat Diet-Induced Mice[J]. Nutrients, 2023, 15(23): 4978.(IF 5.9)

[8] Ji T, Fang B, Zhang M, et al. Succinate Enhances Lipolysis and Decreases Adipocytes Size in Both Subcutaneous and Visceral Adipose Tissue from High-Fat-Diet-Fed Obese Mice[J]. Foods, 2023, 12(23): 4285.(IF 5.2)

[9] Xia K, Chen Y, Liu F, et al. Adaptive responses of erythritol-producing Yarrowia lipolytica to thermal stress after evolution[J]. Applied Microbiology and Biotechnology, 2024, 108(1): 263. (IF 5.0)

[10] Ren F, Liu M, Liu Y, et al. Core microbes closely related with the nutrients and flavor of sweet fermented oats (whole grain food) from China[J]. World Journal of Microbiology and Biotechnology, 2023, 39(9): 236.(IF 4.1)

[11] Ren F, Liu M, Tan B. Bacterial diversity and metabolites: Exploring correlations with preservative properties in soybean pastes[J]. Journal of Food Science, 2024. (IF 3.9)

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