①试剂二配制应尽量晚配,可在操作到加入试剂一时,再配制试剂二;
②必须保证每管的震荡频率及时间一致;
③应在30 min内完成测量,测定完成密封好后再丢弃;
④所用试剂多数为有机溶剂,同一支吸头多次吸取会造成体积不准确,建议取液后更换吸头;
⑤若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;
[1] Lin Y, Liu R, Huang Y, et al. Reactivation of PPARα alleviates myocardial lipid accumulation and cardiac dysfunction by improving fatty acid β-oxidation in Dsg2-deficient arrhythmogenic cardiomyopathy[J]. Acta Pharmaceutica Sinica B, 2023, 13(1): 192-203.(IF 14.903)
[2] Tao T, Shu Q, Zhao Y, et al. Mechanical regulation of lipid and sugar absorption by Piezo1 in enterocytes[J]. Acta Pharmaceutica Sinica B, 2024. (IF 14.5)
[3] Wang W, Zhong Z, Peng S, et al. “All-in-one” metal polyphenol network nanocapsules integrated microneedle patches for lipophagy fueled ferroptosis-mediated multimodal therapy[J]. Journal of Controlled Release, 2024, 373: 599-616. (IF 10.5)
[4] Jinyang W, Yaxin W, Wentao Z, et al. Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stresses[J]. eLife, 2023, 12.(IF 7.7)
[5] Liu H, Ju A, Dong X, et al. Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet β cells in mice[J]. Journal of Translational Medicine, 2023, 21(1): 89.(IF 7.4)
[6] Luo Z, Li M, Yang Q, et al. Ferulic acid prevents nonalcoholic fatty liver disease by promoting fatty acid oxidation and energy expenditure in c57bl/6 mice fed a high-fat diet[J]. Nutrients, 2022, 14(12): 2530.(IF 6.706)
[7] Ji T, Fang B, Wu F, et al. Diet Change Improves Obesity and Lipid Deposition in High-Fat Diet-Induced Mice[J]. Nutrients, 2023, 15(23): 4978.(IF 5.9)
[8] Ji T, Fang B, Zhang M, et al. Succinate Enhances Lipolysis and Decreases Adipocytes Size in Both Subcutaneous and Visceral Adipose Tissue from High-Fat-Diet-Fed Obese Mice[J]. Foods, 2023, 12(23): 4285.(IF 5.2)
[9] Xia K, Chen Y, Liu F, et al. Adaptive responses of erythritol-producing Yarrowia lipolytica to thermal stress after evolution[J]. Applied Microbiology and Biotechnology, 2024, 108(1): 263. (IF 5.0)
[10] Ren F, Liu M, Liu Y, et al. Core microbes closely related with the nutrients and flavor of sweet fermented oats (whole grain food) from China[J]. World Journal of Microbiology and Biotechnology, 2023, 39(9): 236.(IF 4.1)
[11] Ren F, Liu M, Tan B. Bacterial diversity and metabolites: Exploring correlations with preservative properties in soybean pastes[J]. Journal of Food Science, 2024. (IF 3.9)