Acid Phosphatase (ACP) Activity Assay Kit
酸性磷酸酶活性检测试剂盒
货号:AKFA017C
规格: 70T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
Acid Phosphatase (ACP) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:2 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
酸性磷酸酶在酸性环境中能够催化磷酸苯二钠生成游离酚,酚与4-氨基安替比林和铁氰化钾反应生成红色亚醌衍生物,产物在510 nm处具有特征吸收峰,通过吸光值增加速率即可表征酸性磷酸酶的活性。
  • 检测方法: 磷酸苯二钠法
  • 检测波长: 510 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Phenol
  • 参考标准: y=0.1381x+0.0317 (R2=0.9997)
  • 标准线性范围: 0.5-8.0 μmol/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①加入试剂三后必须立即混匀,否则显色不完全;

②待测样本需当天制备且完成检测;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Cui Y, Wang Z, Zhang G, et al. Transmission of nanoplastics from Culex quinquefasciatus to Pardosa pseudoannulata and its impact on predators[J]. Science of The Total Environment, 2022, 820: 153331.(IF 10.753)

[2] Yuan J, Yan R, Zhang X, et al. Soil organic phosphorus is mainly hydrolyzed via phosphatases from ectomycorrhiza-associated bacteria rather than ectomycorrhizal fungi[J]. Plant and Soil, 2024: 1-20. (IF 4.9)

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