Alkaline Phosphatase (AKP/ALP) Activity Assay Kit
碱性磷酸酶活性检测试剂盒
货号:AKFA018C
规格: 70T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
Alkaline Phosphatase (AKP/ALP) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:2 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
碱性磷酸酶在碱性环境中能够催化磷酸苯二钠生成游离酚,进一步与4-氨基安替比林和铁氰化钾反应生成红色亚醌衍生物,产物在510 nm处具有特征吸收峰,通过吸光值变化即可表征碱性磷酸酶的活性。
  • 检测方法: 磷酸苯二钠法
  • 检测波长: 510 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Phenol
  • 参考标准: y=0.2849x-0.0004 (R2=0.9997)
  • 标准线性范围: 0.25-4.0 μmol/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①反应过程中加入试剂三后必须立即充分混匀,否则会导致显色不完全;

②粗酶液制备完成后需置于冰上放置,且当天完成活性检测;

③若测定吸光值超出标准吸光值线性范围:高于最高值建议将粗酶液适当稀释后再进行测定,低于最低值建议适当增加样本量或延长反应时间后再进行测定,计算时相应修改;

④试剂三颜色应为黄色,变为蓝绿色则已失效应停止使用;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhao Z, Li R, Ruan H, et al. Biological signal integrated microfluidic hydrogel microspheres for promoting bone regeneration[J]. Chemical Engineering Journal, 2022, 436: 135176.(IF 16.744)

[2] Chen Q, Wang X, Zhang Y, et. al. Minimizing the ratio of ionizable lipid in lipid nanoparticles for in vivo base editing[J]. National Science Review, 2024, 11:nwae135.(IF 16.3)

[3] Li X, Liang H, Wu Z, et al. Applying a microbial electrolysis cell to promote imidacloprid removal in the adsorption biological coupling system at low temperature via accelerating coke regeneration and bioaugmentation[J]. Chemical Engineering Journal, 2023, 477: 147173.(IF 15.1)

[4] Zhuo T, Wan Q, Chai B, et al. Microbial pathways in the coupling of iron, sulfur, and phosphorus cycles at the sediment–water interface of a river system: An in situ study involving the DGT technique[J]. Science of The Total Environment, 2023, 863: 160855.(IF 10.753)

[5] Cui Y, Wang Z, Zhang G, et al. Transmission of nanoplastics from Culex quinquefasciatus to Pardosa pseudoannulata and its impact on predators[J]. Science of The Total Environment, 2022, 820: 153331.(IF 10.753)

[6] Zhou R, Wang H, Zhang J, et al. Composted maize straw under fungi inoculation reduces soil N2O emissions and mitigates the microbial N limitation in a wheat upland[J]. Science of The Total Environment, 2024: 175728. (IF 8.2)

[7] Chen J, Wan S, Fu Y, et al. An implantable composite scaffold for amplified chemodynamic therapy and tissue regeneration[J]. Journal of Materials Chemistry B, 2023, 11(14): 3151-3163.(IF 7)

[8] Meng Z, Tan M, Azarakhsh N, et al. Antibacterial activity and improvement of the oxidative stability of soybean oil by 4-hydroxybenzyl isothiocyanate from white mustard seeds[J]. Current Research in Food Science, 2023, 7: 100563.(IF 6.3)

[9] Zheng P, Deng J, Jiang L, et al. Polyacrylic acid-reinforced organic-inorganic composite bone adhesives with enhanced mechanical properties and controlled degradability[J]. Journal of Materials Chemistry B, 2024.(IF 6.1)

[10] Deng S, Yang W, Fang C, et al. New insights into the mechanisms of iron absorption: Iron dextran uptake in the intestines of weaned pigs through glucose transporter 5 (GLUT5) and divalent metal transporter 1 (DMT1) transporters[J]. Animal Nutrition, 2024. (IF 6.1)

[11] Yuan J, Yan R, Zhang X, et al. Soil organic phosphorus is mainly hydrolyzed via phosphatases from ectomycorrhiza-associated bacteria rather than ectomycorrhizal fungi[J]. Plant and Soil, 2024: 1-20. (IF 4.9)

[12] Yu B, Li Q, Sun X, et al. O‐GlcNAcylation of TLR4 inhibits osteogenic differentiation of periodontal ligament stem cells[J]. Journal of Periodontal Research, 2023.(IF 3.5)

[13] Zhang Y, Li S, Liang R, et al. A turn-on chemiluminescent assay for alkaline phosphatase using two-dimensional Fe-centered metal–organic frameworks as the signaling probe[J]. Analytical Sciences, 2023: 1-10.(IF 1.6)

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