①试剂二和试剂三均需现用现配且避光保存,配制后有效期较短,为便于试验安排,均附赠一瓶作为备用;
②若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本使用试剂一适当稀释后再进行测定;低于最低值建议适当调整样本量和提取液比例后再进行测定,计算时相应调整;
③脯氨酸和羟脯氨酸与茚三酮反应在570 nm处无吸收峰,测定结果不含这两种氨基酸的含量;
④提取过程会使蛋白变性,若使用蛋白浓度计算,需单独使用PBS提取后再测定蛋白浓度;
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