Glutamic Acid (Glu) Content Assay Kit
谷氨酸含量检测试剂盒
货号:AKAM002M
规格: 120T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Glutamic Acid (Glu) Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:3 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
谷氨酸脱氢酶(GDH)能够催化谷氨酸和NAD生成α-酮戊二酸、NADH和NH4+,NADH可通过PMS的递氢作用,还原WST-1生成甲臜(Formazan),产物在450 nm处具有特征吸收峰,通过吸光值变化即可定量检测谷氨酸的含量。
  • 检测方法: WST-1法
  • 检测波长: 450 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Glutamic Acid
  • 参考标准: y =1.7862x+0.028 (R2=0.999)
  • 标准线性范围: 0.02-0.64 μmol/mL
  • 检测限: 0.02 μmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若A测定或∆A测定超出标准吸光值线性范围:高于最高值建议将待测样本使用蒸馏水适当稀释后再进行测定;低于最低值建议制备更高浓度样本后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Cao J, Yu X, Liu J, et al. Ruxolitinib improves the inflammatory microenvironment, restores glutamate homeostasis, and promotes functional recovery after spinal cord injury[J]. Neural Regeneration Research, 10.4103.(IF 6.1)

[2] Liang T, Yuan Z, Fu L, et al. Integrative transcriptomic and proteomic analysis reveals an alternative molecular network of glutamine synthetase 2 corresponding to nitrogen deficiency in rice (Oryza sativa l.)[J]. International Journal of Molecular Sciences, 2021, 22(14): 7674.(IF 5.924)

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