Proline (Pro) Content Assay Kit
脯氨酸含量检测试剂盒
货号:AKAM003M
规格: 120T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Proline (Pro) Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    冰乙酸(C2H4O2,MW=60.05,CAS:64-19-7);
    甲苯(C7H8,MW=92.14,CAS:108-88-3);
Detection Principle
脯氨酸经磺基水杨酸提取后,与酸性茚三酮反应生成红色物质,产物经甲苯萃取,通过测定520 nm处吸光值变化即可定量检测脯氨酸的含量。
  • 检测方法: 酸性茚三酮法
  • 检测波长: 520 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Proline
  • 参考标准: y =0.0432x+0.0273 (R2=0.9997)
  • 标准线性范围: 1.25-30 μg/mL
  • 检测限: 1 μg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①提取液中含有蛋白沉淀剂,若采用蛋白浓度计算结果,需单独使用PBS提取后再测定蛋白浓度;

②若A测定或∆A测定超出标准吸光值线性范围:高于最高值建议将待测样本使用提取液适当稀释后再进行测定,低于最低值建议适当调整样本量和比例重新提取后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Yuan H, Cheng M, Wang R, et al. miR396b/GRF6 module contributes to salt tolerance in rice[J]. Plant Biotechnology Journal, 2024. (IF 13.8)

[2] Zhang C, Chen X, Han M, et al. Revealing the role of microalgae-bacteria niche for boosting wastewater treatment and energy reclamation in response to temperature[J]. Environmental Science and Ecotechnology, 2023, 14: 100230.(IF 9.371)

[3] Zhao Y, Li Y, Jin Y, et al. The inhibitory effects of Ulva prolifera extracts on early growth of Spartina alterniflora and the underlying mechanisms[J]. Journal of Environmental Management, 2022, 319: 115639.(IF 8.91)

[4] Ye S, Huang Y, Ma T, et al. BnaABF3 and BnaMYB44 regulate the transcription of zeaxanthin epoxidase genes in carotenoid and abscisic acid biosynthesis[J]. Plant Physiology, 2024: kiae184. (IF 7.4)

[5] Liu Y, Yan Y, Ma L, et al. Physiological and metabolomics analyses reveal the resistance response mechanism to tea aphid infestation in new shoots of tea plant (Camellia sinensis)[J]. Plant Stress, 2024: 100545. (IF 6.8)

[6] Li X, Chen L, Liu T, et al. Integrated analysis of ATAC-seq and transcriptomic reveals the ScDof3-ScproC molecular module regulating the cold acclimation capacity of potato[J]. Plant Physiology and Biochemistry, 2024: 108576. (IF 6.5)

[7] Weng W, Lu X, Zhou M, et al. FtbZIP12 Positively Regulates Responses to Osmotic Stress in Tartary Buckwheat[J]. International Journal of Molecular Sciences, 2022, 23(21): 13072.(IF 6.208)

[8] Ye F, Jiang M, Zhang P, et al. Exogenous melatonin reprograms the rhizosphere microbial community to modulate the responses of barley to drought stress[J]. International Journal of Molecular Sciences, 2022, 23(17): 9665.(IF 6.208)

[9] Liu Z, Hu Y, Du A, et al. Cell Wall Matrix Polysaccharides Contribute to Salt–Alkali Tolerance in Rice[J]. International Journal of Molecular Sciences, 2022, 23(23): 15019.(IF 6.208)

[10] Liu N, Jacquemyn H, Liu Q, et al. Effects of a dark septate fungal endophyte on the growth and physiological response of seedlings to drought in an epiphytic orchid[J]. Frontiers in Microbiology, 2022, 13: 961172.(IF 6.064)

[11] Jiang Z, Du L, Shen L, et al. Genome-Wide Exploration and Expression Analysis of the CNGC Gene Family in Eggplant (Solanum melongena L.) under Cold Stress, with Functional Characterization of SmCNGC1a[J]. International Journal of Molecular Sciences, 2023, 24(17): 13049.(IF 5.6)

[12] Song X, Hou X, Zeng Y, et al. Genome-wide identification and comprehensive analysis of WRKY transcription factor family in safflower during drought stress[J]. Scientific Reports, 2023, 13(1): 16955.(IF 4.6)

[13] Li Z, Li X, He F. Drip Irrigation Depth Alters Root Morphology and Architecture and Cold Resistance of Alfalfa[J]. Agronomy, 2022, 12(9): 2192.(IF 3.949)

[14] Yao X, Zhou M, Ruan J, et al. Pretreatment with H2O2 alleviates the negative impacts of NaCl stress on seed germination of Tartary buckwheat (Fagopyrum tataricum)[J]. Plants, 2021, 10(9): 1784.(IF 3.935)

[15] Liu Y, Zhou J, Chen Y, et al. GmSTK12 Participates in Salt Stress Resistance in Soybean[J]. Agronomy, 2023, 13(2): 613.(IF 3.7)

[16] Zhang L, Zhang R, Ye X, et al. Overexpressing VvWRKY18 from grapevine reduces the drought tolerance in Arabidopsis by increasing leaf stomatal density[J]. Journal of Plant Physiology, 2022, 275: 153741.(IF 3.686)

[17] Hou S, Liu Z, Li Y, et al. Exogenous salicylic acid enhanced resistance of Foxtail Millet (Setaria italica) to Sclerospora graminicola[J]. Plant Growth Regulation, 2023, 99(1): 35-44.(IF 3.242)

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