Glutamic Pyruvic Transaminase (GPT) Activity Assay Kit
谷丙转氨酶活性检测试剂盒
货号:AKAM006M
规格: 120T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Glutamic Pyruvic Transaminase (GPT) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
谷丙转氨酶催化丙氨酸和α-酮戊二酸发生转氨基反应,生成丙酮酸和谷氨酸,酮酸中羰基能够与2,4-二硝基苯肼反应生成丙酮酸苯腙,苯腙在碱性条件下呈红棕色,产物在505 nm处具有特征吸收峰,通过吸光值的变化即可表征谷丙转氨酶的活性。
  • 检测方法: 2,4-二硝基苯肼法
  • 检测波长: 505 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Sodium Pyruvate
  • 参考标准: y=0.4005x+0.0336 (R2=0.9993)
  • 标准线性范围: 0.05-1.6 μmol/mL
  • 检测限: -
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若测定吸光值超出标准线性吸光范围:高于最高值建议将粗酶液适当稀释后再进行测定;低于最低值建议适当增加样本量后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Chen Q, Wang X, Zhang Y, et. al. Minimizing the ratio of ionizable lipid in lipid nanoparticles for in vivo base editing[J]. National Science Review, 2024, 11:nwae135.(IF 16.3)

[2] Fan Y, Ou Y, Xiao T, et al. Coordination‐Driven Nanomedicine Mitigates One‐Lung Ventilation‐Induced Lung Injury via Radicals Scavenging and Cell Pyroptosis Inhibition[J]. Small, 2024: 2401056. (IF 13.0)

[3] Yu J, Sun H, Yang Y, et al. Sesamolin alleviates nonalcoholic fatty liver disease through modulating gut microbiota and metabolites in high-fat and high-fructose diet-fed mice[J]. International Journal of Molecular Sciences, 2022, 23(22): 13853.(IF 6.208)

[4] Wang M, Jiang Y, Wang S, et al. Yak milk protects against alcohol-induced liver injury in rats[J]. Food & Function, 2023, 14(21): 9857-9871.(IF 6.1)

[5] Yang Y, Li M, Zhu Y, et al. EZH2 inhibition dampens autoantibody production in lupus by restoring B cell immune tolerance[J]. International Immunopharmacology, 2023, 119: 110155.(IF 5.6)

[6] Lin Z, Li Y, Wang M, et al. Protective effects of yeast extract against alcohol-induced liver injury in rats[J]. Frontiers in Microbiology, 2023, 14.(IF 5.2)

[7] Zhu S, Wang X, Liu G. The Protective Effects of Ganoderma lucidum Active Peptide GLP4 on Lung Injury Induced by Cadmium Poisoning in Mice[J]. Toxics, 2024, 12(6): 378. (IF 4.6)

[8] Du P, Song J, Qiu H, et al. Polyphenols Extracted from Shanxi-Aged Vinegar Inhibit Inflammation in LPS-Induced RAW264. 7 Macrophages and ICR Mice via the Suppression of MAPK/NF-κB Pathway Activation[J]. Molecules, 2021, 26(9): 2745.(IF 4.412)

[9] Peng J, Liang G, Li Y, et al. Identification of a novel FOXO3 agonist that protects against alcohol induced liver injury[J]. Biochemical and Biophysical Research Communications, 2024: 149690. (IF 3.1)

[10] Cao Y, Li S, Zhang Z, et al. A metabolomics study on the mechanisms of Gardeniae fructus against α‐naphthylisothiocyanate‐induced cholestatic liver injury[J]. Biomedical Chromatography, e5961. (IF 1.8)

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