Glutaminase (GLS) Activity Assay Kit
谷氨酰胺酶活性检测试剂盒
货号:AKAM007M
规格: 100T/48S
检测设备:酶标仪
可检测样本数:48 Samples
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Product Information
Glutaminase (GLS) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:48 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
谷氨酰胺酶催化谷氨酰胺生成NH3-N,能够与次氯酸盐和苯酚反应生成蓝色靛酚,产物在630 nm处具有特征吸收峰,通过吸光值变化即可检测氨的含量,进而表征谷氨酰胺酶的活性。
  • 检测方法: 靛酚蓝比色法
  • 检测波长: 630 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Nitrogen
  • 参考标准: To Be Updated
  • 标准线性范围: To Be Updated
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①试剂二配置后尽快使用,若发现变色则停止使用重新配置;

②若测定吸光值大于0.8时,建议将待测样本使用提取液进一步稀释后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Wang X, Yuan L, Lu B, et al. Glutathione promotes the synergistic effects of venetoclax and azacytidine against myelodysplastic syndrome‑refractory anemia by regulating the cell cycle[J]. Experimental and Therapeutic Medicine, 2023, 26(6): 1-11.(IF 2.7)

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