Glutamine Synthetase (GS) Activity Assay Kit
谷氨酰胺合成酶活性检测试剂盒
货号:AKAM008M
规格: 110T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Glutamine Synthetase (GS) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
谷氨酰胺合成酶在ATP和Mg2+存在下,催化铵离子和谷氨酸合成谷氨酰胺,谷氨酰胺进一步转化为γ-谷氨酰基异羟肟酸,在酸性条件下与铁形成红色的络合物,产物在540 nm处具有特征吸收峰,通过吸光值的变化即可表征谷氨酰胺合成酶的活性。
  • 检测方法: 氯化铁比色法
  • 检测波长: 540 nm
  • 信号响应: 递增型
Notices
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhou P, Jiang Y, Adeel M, et al. Nickel Oxide Nanoparticles Improve Soybean Yield and Enhance Nitrogen Assimilation[J]. Environmental Science & Technology, 2023, 57(19): 7547-7558.(IF 11.4)

[2] Yang L, Han R, Duan Y, et al. Exogenous application of silicon and selenium improves the tolerance of tomato plants to calcium nitrate stress[J]. Plant Physiology and Biochemistry, 2024: 108416. (IF 6.5)

[3] Guo Y, Li H, Hao Y, et al. Size Effects of Copper Oxide Nanoparticles on Boosting Soybean Growth via Differentially Modulating Nitrogen Assimilation[J]. Nanomaterials, 2024, 14(9): 746. (IF 5.3)

[4] Jia Z, Zhang J, Jiang W, et al. Different nitrogen concentrations affect strawberry seedlings nitrogen form preferences through nitrogen assimilation and metabolic pathways[J]. Scientia Horticulturae, 2024, 332: 113236. (IF 4.3)

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