Superoxide Dismutase (SOD) Activity Assay Kit
超氧化物歧化酶活性检测试剂盒
货号:AKAO001C-25S
规格: 60T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
Superoxide Dismutase (SOD) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:3 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
黄嘌呤及黄嘌呤氧化酶反应系统能够产生超氧阴离子(O2-),O2-可还原氮蓝四唑生成甲臜,产物在560 nm处具有特征吸收峰;SOD可清除O2-,从而抑制甲臜的形成,反应液颜色越深,表明SOD活性愈低,反之活性越高,通过吸光值的变化即可表征超氧化物歧化酶的活性。
  • 检测方法: XOD-NBT
  • 检测波长: 560 nm
  • 信号响应: 递减型
Notices

①粗酶液和试剂二应用液在测定过程中应置于冰上放置,以免造成变性或失活;

②若测定样本较多时,可将试剂一、试剂二应用液和试剂三按表格中比例配制作为检测工作液,试剂五应用液必须最后加入;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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