①反应过程气泡过多表明酶活性过高,结果可能为负值,建议将粗酶液使用提取液适当稀释后再进行测定;若稀释样本或反应体系没有产生气泡仍然出现较小的负值,说明该样本测不到CAT活性;
②若吸光值超过3,请检查使用的是否96孔UV板;
③若A1大于1.5或ΔA大于0.2,建议将粗酶液使用提取液适当稀释后再进行测定,控制ΔA小于0.2能够确保反应过程曲线呈良好线性,以提高检测准确性和灵敏度;若ΔA小于0.02,建议适当延长酶促反应时间(5-10 min)后再进行测定,计算时相应修改;
④准确在5 s和65 s处完成吸光值测定,以确保结果准确性和重复性;若同时测定多个样本时,应使用多道移液器且分次进行检测,以确保组间反应时间一致;
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