Catalase (CAT) Activity Assay Kit
过氧化氢酶活性检测试剂盒(紫外吸收法)
货号:AKAO003-1U
规格: 60T/50S
检测设备:紫外分光光度计
可检测样本数:50 Samples
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Product Information
Catalase (CAT) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL 石英比色皿(d=10 mm)
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
H2O2在240 nm处具有特征吸收峰,过氧化氢酶能够分解H2O2,使反应溶液240 nm处吸光值随反应时间而下降,根据吸光值变化速率即可表征过氧化氢酶的活性。
  • 检测方法: 紫外吸收法
  • 检测波长: 240 nm
  • 信号响应: 递减型
Notices

①反应过程气泡过多表明酶活性过高,结果可能为负值,建议将粗酶液使用提取液适当稀释后再进行测定;若稀释样本或反应体系没有产生气泡仍然出现较小的负值,说明该样本测不到CAT活性;

②若吸光值超过3,请检查比色皿是否为石英比色皿;

③若A1大于1.5或ΔA大于0.2,建议将粗酶液使用提取液适当稀释后再进行测定,控制ΔA小于0.2能够确保反应过程曲线呈良好线性,以提高检测准确性和灵敏度;若ΔA小于0.02,建议适当延长酶促反应时间(5-10 min)后再进行测定,计算时相应修改;

④准确在5 s和65 s处完成吸光值测定,以确保结果准确性和重复性;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Yang Y Y, Guo H, Huang D W, et al. Simultaneously tuning oxygen reduction pathway and charge transfer dynamics toward sacrificial agent-free photocatalytic H2O2 production for in-situ water disinfection[J]. Chemical Engineering Journal, 2024, 479: 147863.(IF 15.1)

[2] Lu S, Chen Z, Tu H, et al. Multifunctional carbon quantum dots decorated self-healing hydrogel for highly effective treatment of superbug infected wounds[J]. Chemical Engineering Journal, 2023: 148218.(IF 15.1)

[3] Shi L, Zhao X, Zhong K, et al. Physiological mechanism of the response to Cr (VI) in the aerobic denitrifying ectomycorrhizal fungus Pisolithus sp. 1[J]. Journal of Hazardous Materials, 2022, 429: 128318.(IF 14.224)

[4] Yuan H, Cheng M, Wang R, et al. miR396b/GRF6 module contributes to salt tolerance in rice[J]. Plant Biotechnology Journal, 2024. (IF 13.8)

[5] Wu J, Ye W, Feng Y, et al. Aquatic Photolysis of High-Risk Fluorinated Liquid Crystal Monomers: Kinetics, Toxicity Evaluation, and Mechanisms[J]. Water Research, 2024: 121510. (IF 12.8)

[6] Jia S, Diao Y, Li Y, et al. Microbiological interpretation of weak ultrasound enhanced biological wastewater treatment–using Escherichia coli degrading glucose as model system[J]. Bioresource Technology, 2024: 130873. (IF 11.4)

[7] vCui Y, Wang Z, Zhang G, et al. Transmission of nanoplastics from Culex quinquefasciatus to Pardosa pseudoannulata and its impact on predators[J]. Science of The Total Environment, 2022, 820: 153331.(IF 10.753)

[8] Wang S, Ding Y, Xu L, et al. A novel sunflower-like nanocarrier based on dual milk-derived proteins for improved bio-accessibility, stability and antioxidant activity of anthocyanin[J]. Food Hydrocolloids, 2024, 155: 110192. (IF 10.7)

[9] Ji C, Miao J, Xia B, et al. Evaluation of the toxic effects of fluindapyr, a novel SDHI fungicide, to the earthworms Eisenia fetida[J]. Science of The Total Environment, 2023, 899: 165697.(IF 9.8)

[10] Sun J, Yan B, Chen H, et al. Insight into the mechanisms of combined toxicity of cadmium and flotation agents in luminescent bacteria: Role of micro/nano particles[J]. Science of The Total Environment, 2024: 173588. (IF 9.8)

[11] Zhao Y, Li Y, Jin Y, et al. The inhibitory effects of Ulva prolifera extracts on early growth of Spartina alterniflora and the underlying mechanisms[J]. Journal of Environmental Management, 2022, 319: 115639.(IF 8.91)

[12] Chen X, Hu X, Zhang J. Properties of an active film based on chitosan/silk fibroin loaded with an essential oil microemulsion and its application in preservation of strawberries[J]. Food Packaging and Shelf Life, 2024, 43: 101270. (IF 8.0)

[13] Wei Y, Niu S, Xu Y, et al. Removal of dibutyl phthalate (DBP) by bacterial extracellular polymeric substances (EPS) via enzyme catalysis and electron transmission[J]. Journal of Environmental Management, 2024, 368: 122161. (IF 8.0)

[14] Yu W, Cui Y, Han M, et al. Mussel-inspired chemistry in producing mechanically robust and bioactive hydrogels as skin dressings[J]. Materials Today Chemistry, 2023, 27: 101272.(IF 7.613)

[15] Zhang Y, Liu Y, Liu X, et al. Exercise and metformin intervention prevents lipotoxicity-induced hepatocyte apoptosis by alleviating oxidative and ER stress and activating the AMPK/Nrf2/HO-1 signaling pathway in db/db mice[J]. Oxidative Medicine and Cellular Longevity, 2022, 2022.(IF 7.31)

[16] Wang Z, Jiang Q, Li P, et al. The Water Extract of Ampelopsis grossedentata Alleviates Oxidative Stress and Intestinal Inflammation[J]. Antioxidants, 2023, 12(3): 547.(IF 7)

[17] Pu T, Ge Y, Wu M, et al. Effects of black-odorous water bodies on the physiological ecology of Vallisneria natans and its attached biofilm microbial community[J]. Journal of Water Process Engineering, 2024, 63: 105529. (IF 7.0)

[18] Zhu K, Yan D, Wang Y, et al. Chlorophyll retention reduces storability and pathogen defense in a novel citrus brown flavedo mutant[J]. Postharvest Biology and Technology, 2022, 192: 112006.(IF 6.751)

[19] Xiang X, Wang H, Zhou W, et al. Glutathione Protects against Paraquat-Induced Oxidative Stress by Regulating Intestinal Barrier, Antioxidant Capacity, and CAR Signaling Pathway in Weaned Piglets[J]. Nutrients, 2023, 15(1): 198.(IF 6.706)

[20] Yuan P, Shen W, Yang L, et al. Physiological and transcriptional analyses reveal the resistance mechanisms of kiwifruit (Actinidia chinensis) mutant with enhanced heat tolerance[J]. Plant Physiology and Biochemistry, 2024, 207: 108331.(IF 6.5)

[21] Weng W, Lu X, Zhou M, et al. FtbZIP12 Positively Regulates Responses to Osmotic Stress in Tartary Buckwheat[J]. International Journal of Molecular Sciences, 2022, 23(21): 13072.(IF 6.208)

[22] Yao X, Zhou M, Ruan J, et al. Physiological and Biochemical Regulation Mechanism of Exogenous Hydrogen Peroxide in Alleviating NaCl Stress Toxicity in Tartary Buckwheat (Fagopyrum tataricum (L.) Gaertn)[J]. International Journal of Molecular Sciences, 2022, 23(18): 10698.(IF 6.208)

[23] Chen X L, Wu L J, Miao L L, et al. Chronic polystyrene microplastics exposure-induced changes in thick-shell mussel (Mytilus coruscus) metaorganism: A holistic perspective[J]. Ecotoxicology and Environmental Safety, 2024, 284: 116961. (IF 6.2)

[24] Shu P, Li Y, Sheng J, et al. Tomato SlMAPK3 Modulates Cold Resistance by Regulating the Synthesis of Raffinose and the Expression of SlWRKY46[J]. Journal of Agricultural and Food Chemistry, 2024. (IF 6.1)

[25] Li C, Cui Z, Deng S, et al. Programmed Cell Death Protein 2-like Promotes Inflammation and Oxidative Stress in Vascular Endothelial Cells[J]. ACS Pharmacology & Translational Science, 2023, 6(10): 1453-1470.(IF 6)

[26] Li J, Cai B, Chang S, et al. Mechanisms associated with the synergistic induction of resistance to tobacco black shank in tobacco by arbuscular mycorrhizal fungi and β-aminobutyric acid[J]. Frontiers in Plant Science, 2023, 14.(IF 5.6)

[27] Wang X, Tang Y, Li Z, et al. Investigation of Immune Responses in Giant African Snail, Achatina immaculata, against a Two-Round Lipopolysaccharide Challenge[J]. International Journal of Molecular Sciences, 2023, 24(15): 12191.(IF 5.6)

[28] Zhen X, Liu C, Guo Y, et al. Leaf Senescence Regulation Mechanism Based on Comparative Transcriptome Analysis in Foxtail Millet[J]. International Journal of Molecular Sciences, 2024, 25(7): 3905. (IF 5.6)

[29] Li J, Liu X, Ahmad N, et al. CePP2C19 confers tolerance to drought by regulating the ABA sensitivity in Cyperus esculentus[J]. BMC Plant Biology, 2023, 23(1): 524.(IF 5.3)

[30] Yang Z, Wang F, Yin Y, et al. Dietary Litsea cubeba essential oil supplementation improves growth performance and intestinal health of weaned piglets[J]. Animal Nutrition, 2023, 13: 9-18.(IF 5.285)

[31] Xia K, Chen Y, Liu F, et al. Adaptive responses of erythritol-producing Yarrowia lipolytica to thermal stress after evolution[J]. Applied Microbiology and Biotechnology, 2024, 108(1): 263. (IF 5.0)

[32] Liu L, Shi M, Wu Y, et al. Interactive effects of dinotefuran and Nosema ceranae on the survival status and gut microbial community of honey bees[J]. Pesticide Biochemistry and Physiology, 2024, 200: 105808. (IF 4.7)

[33] Song X, Hou X, Zeng Y, et al. Genome-wide identification and comprehensive analysis of WRKY transcription factor family in safflower during drought stress[J]. Scientific Reports, 2023, 13(1): 16955.(IF 4.6)

[34] He Q, Jiang Y, Huang C, et al. Molecular mechanism of delayed development by interfering RNA targeting the phenylalanine ammonia lyase gene (pal1) in Pleurotus ostreatus[J]. Journal of Integrative Agriculture, 2024. (IF 4.6)

[35] Ding M, Xing W, Li Z, et al. The class B heat shock factor RcHsf17 from Rosa chinensis enhances basal thermotolerance in Rosa rugosa[J]. Environmental and Experimental Botany, 2024: 105832. (IF 4.5)

[36] Zheng X, Yuan Z, Yu Y, et al. OsCSD2 and OsCSD3 Enhance Seed Storability by Modulating Antioxidant Enzymes and Abscisic Acid in Rice[J]. Plants, 2024, 13(2): 310.(IF 4.5)

[37] Xu J, Kang Z, Zhu K, et al. RBOH1-dependent H2O2 mediates spermine-induced antioxidant enzyme system to enhance tomato seedling tolerance to salinity–alkalinity stress[J]. Plant Physiology and Biochemistry, 2021, 164: 237-246.(IF 4.27)

[38] Wang G, Zhang X, Du G, et al. Oleic Acid and Linoleic Acid Enhances the Biocontrol Potential of Metarhizium rileyi[J]. Journal of Fungi, 2024, 10(8): 521. (IF 4.2)

[39] Zhang H, Xiang X, Wang C, et al. Different effects of acute and chronic oxidative stress on the intestinal flora and gut-liver axis in weaned piglets[J]. Frontiers in Microbiology, 2024, 15: 1414486. (IF 4.0)

[40] Li Z, Li X, He F. Drip Irrigation Depth Alters Root Morphology and Architecture and Cold Resistance of Alfalfa[J]. Agronomy, 2022, 12(9): 2192.(IF 3.949)

[41] Yao X, Zhou M, Ruan J, et al. Pretreatment with H2O2 alleviates the negative impacts of NaCl stress on seed germination of Tartary buckwheat (Fagopyrum tataricum)[J]. Plants, 2021, 10(9): 1784.(IF 3.935)

[42] Liu J, Zuo X, Bi J, et al. Palliative Effect of Combined Application of Zinc and Selenium on Reproductive Injury Induced by Tripterygium Glycosides in Male Rats[J]. Biological Trace Element Research, 2024: 1-13.(IF 3.9)

[43] Wu H, Wang X, Gao H, et al. Alleviating Cd Stress in Sunflower (Helianthus annuus) through the Sodium Silicate Application[J]. Sustainability, 2024, 16(5): 2037. (IF 3.9)

[44] Li L, Wu S, Wang S, et al. Molecular Mechanism of Exogenous Selenium Affecting the Nutritional Quality, Species and Content of Organic Selenium in Mustard[J]. Agronomy, 2023, 13(5): 1425.(IF 3.7)

[45] Liu Y, Zhou J, Chen Y, et al. GmSTK12 Participates in Salt Stress Resistance in Soybean[J]. Agronomy, 2023, 13(2): 613.(IF 3.7)

[46] Zhang L, Zhang R, Ye X, et al. Overexpressing VvWRKY18 from grapevine reduces the drought tolerance in Arabidopsis by increasing leaf stomatal density[J]. Journal of Plant Physiology, 2022, 275: 153741.(IF 3.686)

[47] Du L, Peng X, Zhang H, et al. Transcriptome Analysis and QTL Mapping Identify Candidate Genes and Regulatory Mechanisms Related to Low-Temperature Germination Ability in Maize[J]. Genes, 2023, 14(10): 1917.(IF 3.5)

[48] Wang F H, Zhang C, Wang C L, et al. Estimating the role of maize Y-EPSPS gene in glyphosate resistance in Arabidopsis transgenic lines[J]. Plant Growth Regulation, 2024: 1-13. (IF 3.5)

[49] Cheng H, Shi X, Li L. The Effect of Exogenous Selenium Supplementation on the Nutritional Value and Shelf Life of Lettuce[J]. Agronomy, 2024, 14(7): 1380. (IF 3.3)

[50] Cheng H, Chang S, Shi X, et al. Molecular Mechanisms of the Effects of Sodium Selenite on the Growth, Nutritional Quality, and Species of Organic Selenium in Dandelions[J]. Horticulturae, 2024, 10(3): 209. (IF 3.1)

[51] Hu Z, Wang S, Wang Y, et al. Genome-Wide Identification of DREB Transcription Factor Family and Functional Analysis of PaDREB1D Associated with Low-Temperature Stress in Phalaenopsis aphrodite[J]. Horticulturae, 2024, 10(9): 933. (IF 3.1)

[52] Li Q Y, Wang S, Wu G Y, et al. Physiological and biochemical changes in leaf abscission of Cyclocarya paliurus stem segments in vitro[J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2023: 1-11.(IF 3)

[53] Li X, Xiu D, Huang J, et al. Nutshell Physicochemical Characteristics of Different Hazel Cultivars and Their Defensive Activity toward Curculio nucum (Coleoptera: Curculionidae)[J]. Forests, 2023, 14(2): 319.(IF 2.9)

[54] Liang Y E, Zhang H, Zhu J, et al. Transcriptomic Analysis Reveals the Involvement of Flavonoids Synthesis Genes and Transcription Factors in Dracaena cambodiana Response to Ultraviolet-B Radiation[J]. Forests, 2023, 14(5): 979.(IF 2.9)

[55] Yang X, Wang Z, Zhang Y, et al. Reducing the injury of hippocampal vascular endothelial cells after stroke via targeting SIRT1 by butylphthalide[J]. Materials Express, 2024, 14(5): 762-769. (IF 0.7)

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