Polyphenol Oxidase (PPO) Activity Assay Kit
多酚氧化酶活性检测试剂盒
货号:AKAO004C-25S
规格: 60T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
Polyphenol Oxidase (PPO) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:3 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
多酚氧化酶能够催化邻苯二酚生成醌,产物在410 nm处具有特征吸收峰,通过吸光值变化即可表征多酚氧化酶的活性。
  • 检测方法: 邻苯二酚法
  • 检测波长: 410 nm
  • 信号响应: 递增型
Notices

①不同样本的多酚氧化酶最佳反应温度略有差别,反应温度可在25-37℃之间进行调整;

②若A测定大于1.5或ΔA大于1.0,建议将粗酶液适当稀释后再进行测定;若ΔA小于0.02,建议适当延长酶促反应时间或适当增加样本量后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Cui Y, Wang Z, Zhang G, et al. Transmission of nanoplastics from Culex quinquefasciatus to Pardosa pseudoannulata and its impact on predators[J]. Science of The Total Environment, 2022, 820: 153331.(IF 10.753)

[2] Liu Y, Yan Y, Ma L, et al. Physiological and metabolomics analyses reveal the resistance response mechanism to tea aphid infestation in new shoots of tea plant (Camellia sinensis)[J]. Plant Stress, 2024: 100545. (IF 6.8)

[3] Ouyang W, Ning J, Zhu X, et al. UPLC-ESI-MS/MS analysis revealed the dynamic changes and conversion mechanism of non-volatile metabolites during green tea fixation[J]. LWT, 2024: 116010. (IF 6.0)

[4] Li G, Mo Y, Lv J, et al. Unraveling verticillium wilt resistance: insight from the integration of transcriptome and metabolome in wild eggplant[J]. Frontiers in Plant Science, 2024, 15: 1378748. (IF 5.6)

[5] Li X, Xiu D, Huang J, et al. Nutshell Physicochemical Characteristics of Different Hazel Cultivars and Their Defensive Activity toward Curculio nucum (Coleoptera: Curculionidae)[J]. Forests, 2023, 14(2): 319.(IF 2.9)

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